Mechanical Conditioning Influences the Metabolic Response of Cell-Seeded Constructs
Mechanical Conditioning Influences the Metabolic Response of Cell-Seeded Constructs
复制标题
机械调节影响细胞接种结构的代谢反应
作者：
J. Shelton;D. Bader;D. Lee
承重软组织的损伤和退行性疾病在医院诊所极为常见，涉及所有年龄段的人群。由于移植物和基于假体的策略的相对失败，组织工程为该领域的发展提供了一个主要焦点。在植入时，这种系统将受到正常的生理力。因此，重要的是要了解修复系统内细胞的机械调节的影响，以预测它们的反应和最终的成功在体内。在目前的研究中，两个良好的表征模型系统，涉及真皮成纤维细胞接种到硅胶膜和软骨细胞接种在琼脂糖结构，已被用来研究机械负荷对细胞活性的影响。所呈现的数据表明，单层成纤维细胞的拉伸调节和三维琼脂糖构建体中软骨细胞的压缩负荷产生了不同的反应。特别是，与拉伸系统相关的应变场的性质在确定最终单元响应中是重要的。同样，在压缩系统中，动态频率的影响也很重要。决定对这两种类型的条件作用的反应的其他关键因素与细胞的年龄及其在组织中的原始位置有关。
Injuries and degenerative diseases to load-bearing soft tissues are extremely common in hospital clinics and involve all ages of the population. Tissue engineering provides a major focus for developments within this area due to the relative failure of graft and prosthetic-based strategies. On implantation, such systems will be subjected to normal physiological forces. It is important, therefore, to understand the effects of mechanical conditioning of cells within repair systems to predict their response and ultimate success in vivo. In the current study two well-characterised model systems, involving dermal fibroblasts seeded onto silicone membranes and chondrocytes seeded in agarose constructs, have been used to study the effects of mechanical loading on cellular activity. The data presented demonstrate that both the tensile conditioning of fibroblasts in monolayer and the compressive loading of chondrocytes in three-dimensional agarose constructs produced distinct responses. In particular, the nature of the strain field associated with the tensile system is important in determining the ultimate cell response. Equally within the compressive system the influence of dynamic frequency was shown to be important. The other critical factors in determining response to both types of conditioning are associated with the age of the cell and its original location within the tissue.
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应力剥夺对内侧副韧带胶原蛋白代谢更新的影响。
影响因子：
4.2
作者：
Amiel,D;Akeson,WH;Harwood,FL;Frank,CB
通讯作者：
Frank,CB
影响因子：
4
作者：
A. Banes;J. Gilbert;D. Taylor;O. Monbureau
通讯作者：
A. Banes;J. Gilbert;D. Taylor;O. Monbureau
DOI：
10.1016/0304-4165(89)90067-6
发表时间：
1989-06-27
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GRAY, ML;PIZZANELLI, AM;SWANN, DA
通讯作者：
SWANN, DA
影响因子：
3.9
作者：
SAH, RLY;DOONG, JYH;SANDY, JD
通讯作者：
SANDY, JD
DOI：
10.1152/ajpcell.1998.274.5.c1424
发表时间：
1998-05-01
影响因子：
5.5
作者：
Gudi, SRP;Lee, AA;Frangos, JA
通讯作者：
Frangos, JA