Effects of transplantation of adipose tissue-derived stem cells on prostate tumor.
Effects of transplantation of adipose tissue-derived stem cells on prostate tumor.
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脂肪组织干细胞移植对前列腺肿瘤的影响。
DOI：
10.1002/pros.21140
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发表时间：
2010-07-01
期刊：
影响因子：
2.8
通讯作者：
Lin, Ching-Shwun
中科院分区：
文献类型：
--
作者：
Lin, Guiting;Yang, Rong;Banie, Lia;Wang, Guifang;Ning, Hongxiu;Li, Long-Cheng;Lue, Tom F.;Lin, Ching-Shwun
肥胖是前列腺癌发生的一个风险因素，但其潜在机制尚不清楚。本研究验证了一个假设，即癌细胞可能募集脂肪组织的基质细胞来促进肿瘤生长。
将PC3前列腺癌细胞移植到无胸腺小鼠右侧腹皮下间隙。一周后，将脂肪组织来源的基质细胞或干细胞（ADSC）或磷酸盐缓冲盐水（PBS，作为对照）以同样方式移植到左侧腹。在接下来的34天内监测肿瘤大小；之后，处死小鼠并摘取肿瘤进行组织学检查。通过迁移实验检测PC3细胞吸引ADSC的能力。通过在特异性抑制剂AMD3100存在的情况下进行迁移实验来检测CXCL12/CXCR4轴的参与情况。
在整个过程中，ADSC处理组小鼠的PC3肿瘤平均大小大于PBS处理组小鼠。在ADSC处理组小鼠的肿瘤内发现了ADSC；还检测到了CXCR4的表达。迁移实验表明CXCL12/CXCR4轴参与了ADSC向PC3细胞的迁移。ADSC处理组小鼠肿瘤中的毛细血管密度是PBS处理组小鼠肿瘤的两倍。ADSC处理组小鼠肿瘤中的血管内皮生长因子（VEGF）表达相似，但成纤维细胞生长因子2（FGF2）表达明显高于PBS处理组小鼠肿瘤。
前列腺癌细胞通过CXCL12/CXCR4轴募集ADSC。ADSC通过增加肿瘤血管生成来促进肿瘤生长，这是由FGF2介导的。
Obesity is a risk factor for prostate cancer development, but the underlying mechanism is unknown. The present study tested the hypothesis that stromal cells of the adipose tissue might be recruited by cancer cells to help tumor growth. PC3 prostate cancer cells were transplanted into the subcutaneous space of the right flank of athymic mice. One week later, adipose tissue-derived stromal or stem cells (ADSC) or phosphate-buffered saline (PBS, as control) was transplanted similarly to the left flank. Tumor size was monitored for the next 34 days; afterwards, the mice were sacrificed and their tumors harvested for histological examination. The ability of PC3 cells to attract ADSC was tested by migration assay. The involvement of the CXCL12/CXCR4 axis was tested by migration assay in the presence of a specific inhibitor AMD3100. Throughout the entire course, the average size of PC3 tumors in ADSC-treated mice was larger than in PBS-treated mice. ADSC were identified inside the tumors of ADSC-treated mice; CXCR4 expression was also detected. Migration assay indicated the involvement of the CXCL12/CXCR4 axis in the migration of ADSC toward PC3 cells. Capillary density was twice as high in the tumors of ADSC-treated mice than in the tumors of PBS-treated mice. VEGF expression was similar but FGF2 expression was significantly higher in tumors of ADSC-treated mice than in the tumors of PBS-tread mice. Prostate cancer cells recruited ADSC by the CXCL12/CXCR4 axis. ADSC helps tumor growth by increasing tumor vascularity, and which was mediated by FGF2.
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影响因子：
5.2
作者：
Lazennec, Gwendal;Jorgensen, Christian
通讯作者：
Jorgensen, Christian
构建不同缺失子表达质粒鉴定肾细胞癌CXCR4核定位序列
影响因子：
2.6
作者：
Wang, Lin-Hui;Liu, Qian;Sun, Ying-Hao
通讯作者：
Sun, Ying-Hao
影响因子：
5.2
作者：
Constantin, Gabriela;Marconi, Silvia;Bonetti, Bruno
通讯作者：
Bonetti, Bruno
影响因子：
4.1
作者：
Meirelles, Lindolfo da Silva;Sand, Theodore T.;Caplan, Arnold I.
通讯作者：
Caplan, Arnold I.
影响因子：
3.8
作者：
Rodriguez, Carmen;Freedland, Stephen J.;Calle, Eugenia E.
通讯作者：
Calle, Eugenia E.