Identification of a unique Radical SAM methyltransferase required for the sp(3)-C-methylation of an arginine residue of methyl-coenzyme M reductase.
Identification of a unique Radical SAM methyltransferase required for the sp(3)-C-methylation of an arginine residue of methyl-coenzyme M reductase.
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DOI：
10.1038/s41598-018-25716-x
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发表时间：
2018-05-09
影响因子：
4.6
通讯作者：
Layer G
中科院分区：
文献类型：
--
作者：
Deobald D;Adrian L;Schöne C;Rother M;Layer G
甲烷的生物形成（甲烷生成）是一个全球重要的过程，在沼气技术中得到利用，但也通过向大气中释放强效温室气体而导致全球变暖。甲烷生成的最后一步也是甲烷释放步骤是由甲基辅酶M还原酶（MCR）催化的，该酶携带几种特殊的翻译后氨基酸修饰。其中，5-C-（S）-甲基精氨酸位于酶的活性位点附近。在这里，我们表明，一个独特的自由基S-腺苷-L-甲硫氨酸（SAM）甲基转移酶是必需的精氨酸残基的甲基化。编码甲基转移酶的基因目前被注释为“甲烷生成标记10”，其功能至今未知。乙酸甲烷八叠球菌WWM 1中甲基转移酶基因ma 4551的缺失导致产生缺乏相应精氨酸残基的C-5-甲基化的活性MCR。研究了相应的M.乙酰化酵母突变菌株的特性和分离的MCR的生物物理特性表明，甲基化精氨酸对于应激条件下MCR的稳定性是重要的。
The biological formation of methane (methanogenesis) is a globally important process, which is exploited in biogas technology, but also contributes to global warming through the release of a potent greenhouse gas into the atmosphere. The last and methane-releasing step of methanogenesis is catalysed by the enzyme methyl-coenzyme M reductase (MCR), which carries several exceptional posttranslational amino acid modifications. Among these, a 5-C-(S)-methylarginine is located close to the active site of the enzyme. Here, we show that a unique Radical S-adenosyl-L-methionine (SAM) methyltransferase is required for the methylation of the arginine residue. The gene encoding the methyltransferase is currently annotated as “methanogenesis marker 10” whose function was unknown until now. The deletion of the methyltransferase gene ma4551 in Methanosarcina acetivorans WWM1 leads to the production of an active MCR lacking the C-5-methylation of the respective arginine residue. The growth behaviour of the corresponding M. acetivorans mutant strain and the biophysical characterization of the isolated MCR indicate that the methylated arginine is important for MCR stability under stress conditions.
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影响因子：
14.9
作者：
Haft DH;Selengut JD;Richter RA;Harkins D;Basu MK;Beck E
通讯作者：
Beck E
DOI：
10.1111/j.1432-1033.1988.tb13941.x
发表时间：
1988-03-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
ELLERMANN, J;HEDDERICH, R;THAUER, RK
通讯作者：
THAUER, RK
影响因子：
5.4
作者：
Kahnt, Joerg;Buchenau, Baerbel;Thauer, Rudolf K.
通讯作者：
Thauer, Rudolf K.
影响因子：
3.2
作者：
Allen, Kylie D.;Xu, Huimin;White, Robert H.
通讯作者：
White, Robert H.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro