Differences in survival, virulence and biofilm formation between sialidase-deficient and W83 wild-type Porphyromonas gingivalis strains under stressful environmental conditions
Differences in survival, virulence and biofilm formation between sialidase-deficient and W83 wild-type Porphyromonas gingivalis strains under stressful environmental conditions
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唾液酸酶缺陷型和 W83 野生型牙龈卟啉单胞菌菌株在应激环境条件下存活率、毒力和生物膜形成的差异
DOI：
10.1186/s12866-017-1087-2
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发表时间：
2017-08
期刊：
影响因子：
4.2
通讯作者：
Li Chen
中科院分区：
文献类型：
--
作者：
Xu Xiaoyu;Tong Tong;Yang Xue;Pan Yaping;Lin Li;Li Chen
背景：牙龈卟啉单胞菌是慢性牙周炎的主要致病菌。在炎症微环境中，存在极端的pH值、升高的温度以及氧化应激。病原体要适应这些充满压力的环境。
BackgroundPorphyromonas gingivalis is a major causative pathogen of chronic periodontitis. Within the inflammatory microenvironment, there exists extreme pH values, elevated temperatures and oxidative stress. Pathogens adapt to these stressful environmental conditions by regulating the transcription of virulence genes, modifying themselves with macromolecules and by aggregating and entering into a biofilm growth phase. Our previous study showed that the P. gingivalis sialidase can help cells obtain sialic acid from the environment, which is used to modify macromolecules on the surface of P. gingivalis cells. In this study, we compared the survival, virulence factors and biofilm formation of a sialidase-deficient strain (ΔPG0352) and the wild-type P. gingivalis W83 strain under various pH values, temperatures and oxidative stress conditions to identify the roles of sialidase in the adaptation of P. gingivalis to stressful conditions.ResultsCompared to the growth of the P. gingivalis W83 strain, the growth of the △PG0352 was more inhibited by oxidative stress (0.25 and 0.5 mM H2O2) and exhibited greater cell structure damage when treated with H2O2 as assessed by transmission electron microscopy. Both Lys-gingipain (Kgp) and Arg-gingipain (Rgp) activities were lower in the ΔPG0352 than those in the P. gingivalis W83 strain under all the assayed culture conditions. The lipopolysaccharide (LPS) activity of the W83 strain was higher than that of the ΔPG0352 under acidic conditions (pH 5.0), but no differences between the strains were observed under other conditions. Compared to the biofilms formed by P. gingivalis W83, those formed by the ΔPG0352 were decreased and discontinuous under acidic, alkaline and oxidative stress conditions.ConclusionCompared to the P. gingivalis W83 strain, the survival, virulence and biofilm formation of the ΔPG0352 were decreased under stressful environmental conditions.
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影响因子：
4.2
作者：
van Dillewijn P;Sanjuán J;Olivares J;Soto MJ
通讯作者：
Soto MJ
影响因子：
3.7
作者：
Bloch S;Thurnheer T;Murakami Y;Belibasakis GN;Schäffer C
通讯作者：
Schäffer C
可切除的结直肠癌：当前对复发风险，微生物群和液体活检中基因突变检测的看法。
影响因子：
5.2
作者：
Koulouris A;Tsagkaris C;Messaritakis I;Gouvas N;Sfakianaki M;Trypaki M;Spyrou V;Christodoulakis M;Athanasakis E;Xynos E;Tzardi M;Mavroudis D;Souglakos J
通讯作者：
Souglakos J
影响因子：
3.7
作者：
Reyes L;Eiler-McManis E;Rodrigues PH;Chadda AS;Wallet SM;Bélanger M;Barrett AG;Alvarez S;Akin D;Dunn WA Jr;Progulske-Fox A
通讯作者：
Progulske-Fox A
影响因子：
5.2
作者：
How KY;Song KP;Chan KG
通讯作者：
Chan KG