Behavior of two Tannerella forsythia strains and their cell surface mutants in multispecies oral biofilms.
Behavior of two Tannerella forsythia strains and their cell surface mutants in multispecies oral biofilms.
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DOI：
10.1111/omi.12182
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发表时间：
2017-10
影响因子：
3.7
通讯作者：
Schäffer C
中科院分区：
文献类型：
--
作者：
Bloch S;Thurnheer T;Murakami Y;Belibasakis GN;Schäffer C
作为龈下多物种生物膜的一员，连翘坦纳菌通常与牙周炎有关。该细菌具有用独特的 O-聚糖修饰的特征细胞表面 (S-) 层。本研究采用模拟口腔情况的体外多物种生物膜模型，分析了 S 层和 O 聚糖在生物膜形成中的作用。将具有细胞表面组成缺陷的不同连翘菌株和突变体以及九种精选口腔细菌纳入该模型。使用菌落形成单位计数和定量实时聚合酶链反应定量分析连翘 S 层和附着的聚糖对生物膜细菌组成的影响，并通过荧光原位杂交和共焦激光扫描显微镜定性分析。这表明，连翘细胞表面的变化不会影响多物种聚生体的数量组成，但直肠弯曲杆菌细胞数量除外。连翘在细菌聚集体中的定位根据 S 层聚糖的变化而变化，这也影响了它与牙龈卟啉单胞菌的聚集。这表明糖基化连翘 S 层对该物种在生物膜内的定位、其与牙龈卟啉单胞菌的共定位以及直连连翘的流行具有选择性作用。这些发现可能转化为连翘细胞表面结构在与生物膜内部或外部的宿主组织和免疫系统相互作用时对该物种的毒力的潜在作用。
As a member of subgingival multispecies biofilms, Tannerella forsythia is commonly associated with periodontitis. The bacterium has a characteristic cell surface (S‐) layer modified with a unique O‐glycan. Both the S‐layer and the O‐glycan were analyzed in this study for their role in biofilm formation by employing an in vitro multispecies biofilm model mimicking the situation in the oral cavity. Different T. forsythia strains and mutants with characterized defects in cell surface composition were incorporated into the model, together with nine species of select oral bacteria. The influence of the T. forsythia S‐layer and attached glycan on the bacterial composition of the biofilms was analyzed quantitatively using colony‐forming unit counts and quantitative real‐time polymerase chain reaction, as well as qualitatively by fluorescence in situ hybridization and confocal laser scanning microscopy. This revealed that changes in the T. forsythia cell surface did not affect the quantitative composition of the multispecies consortium, with the exception of Campylobacter rectus cell numbers. The localization of T. forsythia within the bacterial agglomeration varied depending on changes in the S‐layer glycan, and this also affected its aggregation with Porphyromonas gingivalis. This suggests a selective role for the glycosylated T. forsythia S‐layer in the positioning of this species within the biofilm, its co‐localization with P. gingivalis, and the prevalence of C. rectus. These findings might translate into a potential role of T. forsythia cell surface structures in the virulence of this species when interacting with host tissues and the immune system, from within or beyond the biofilm.
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影响因子：
4.2
作者：
Guggenheim, Bernhard;Gmuer, Rudolf;Kinane, Denis F.
通讯作者：
Kinane, Denis F.
影响因子：
4.2
作者：
Bao K;Belibasakis GN;Thurnheer T;Aduse-Opoku J;Curtis MA;Bostanci N
通讯作者：
Bostanci N
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ
影响因子：
3.1
作者：
GRENIER, D;UITTO, VJ;MCBRIDE, BC
通讯作者：
MCBRIDE, BC
影响因子：
3.8
作者：
Honma, Kiyonobu;Inagaki, Satoru;Sharma, Ashu
通讯作者：
Sharma, Ashu