Detection of human polyomaviruses MCPyV, HPyV6, and HPyV7 in malignant and non‐malignant tonsillar tissues
Detection of human polyomaviruses MCPyV, HPyV6, and HPyV7 in malignant and non‐malignant tonsillar tissues
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恶性和非恶性扁桃体组织中人多瘤病毒 MCPyV、HPyV6 和 HPyV7 的检测
DOI：
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发表时间：
2016
影响因子：
12.7
通讯作者：
V. Šroller
中科院分区：
文献类型：
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作者：
M. Saláková;E. Košlabová;Zuzana Vojtěchová;R. Tachezy;V. Šroller
默克尔细胞多瘤病毒（MCPyV）与默克尔细胞癌（MCC）有关，这是一种罕见的皮肤恶性肿瘤。在皮肤上发现了人类多瘤病毒6和7 (HPyV6和HPyV7)，但未与任何病理相关。血清学资料表明，多瘤病毒感染发生在儿童时期，在人群中广泛存在。然而，持续感染的部位尚未确定。采用qPCR技术对103例福尔马林固定石蜡包埋（FFPE）标本和5例非恶性扁桃体新鲜冷冻组织（FF）以及97例扁桃体癌FFPE和15例扁桃体癌组织进行MCPyV、HPyV6和HPyV7 DNA检测。筛选所有MCPyV DNA阳性FF组织中早期病毒转录物的表达。MCPyV、HPyV6和HPyV7在非恶性扁桃体组织中的总体患病率分别为10.2%、4.6%和0.9%。MCPyV DNA在非恶性扁桃体中的流行率随年龄增加而增加（P < 0.05）。肿瘤组织中MCPyV DNA的患病率明显高于非恶性组织（35.7%比10.2%）（P < 0.001）， HPyV6 DNA的患病率（5.4%比4.6%）和HPyV7 DNA的患病率（1.8%比0.9%）是相当的。在所有MCPyV DNA阳性的FF组织中检测到早期转录本。在扁桃体中发现了MCPyV、HPyV6和HPyV7 dna，提示扁桃体可能是病毒潜伏的一个部位。病毒载量很低，表明只有一小部分细胞被感染。在扁桃体肿瘤中检测到MCPyV DNA的较高流行率，但在肿瘤组织和健康组织之间的病毒载量没有差异。中华检验医学杂志，2016,33(2):391 - 391。©2015 Wiley期刊公司
Merkel cell polyomavirus (MCPyV) is associated with Merkel cell carcinoma (MCC), a rare skin malignancy. Human polyomavirus six and seven (HPyV6 and HPyV7) were identified on a skin but have not been associated with any pathology. The serology data suggest that infection with polyomaviruses occurs in childhood and they are widespread in population. However, the site of persistent infection has not been identified. Altogether, 103 formalin‐fixed paraffin‐embedded (FFPE) specimens and five fresh frozen tissues (FF) of non‐malignant tonsils and 97 FFPE and 15 FF samples of tonsillar carcinomas were analyzed by qPCR for the presence of MCPyV, HPyV6, and HPyV7 DNA. All MCPyV DNA positive FF tissues were screened for the expression of early viral transcripts. Overall prevalence of MCPyV, HPyV6, and HPyV7 in non‐malignant tonsillar tissues was 10.2%, 4.6%, and, 0.9%, respectively. The prevalence of MCPyV DNA in non‐malignant tonsils increased with age (P < 0.05). While the prevalence of MCPyV DNA was significantly higher in the tumors than non‐malignant tissues (35.7% vs. 10.2%) (P < 0.001), the prevalence of HPyV6 DNA (5.4% vs. 4.6%) and HPyV7 DNA (1.8% vs. 0.9%) were comparable. In all MCPyV DNA positive FF tissues early transcripts were detected. MCPyV, HPyV6, and HPyV7 DNAs were found in tonsils, suggesting that the tonsils may be a site of viral latency. The viral load was low indicating that only a fraction of cells are infected. The higher prevalence of MCPyV DNA was detected in tonsillar tumors but there was no difference in the viral load between tumor and healthy tissues. J. Med. Virol. 88:695–702, 2016. © 2015 Wiley Periodicals, Inc.
影响因子：
30.3
作者：
Schowalter RM;Pastrana DV;Pumphrey KA;Moyer AL;Buck CB
通讯作者：
Buck CB
影响因子：
6.4
作者：
Ho, Jonhan;Jedrych, Jaroslaw J.;Chang, Yuan
通讯作者：
Chang, Yuan