Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin.
Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin.
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DOI：
10.1016/j.chom.2010.05.006
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发表时间：
2010-06-25
影响因子：
30.3
通讯作者：
Buck CB
中科院分区：
文献类型：
--
作者：
Schowalter RM;Pastrana DV;Pumphrey KA;Moyer AL;Buck CB
越来越多的证据支持这一概念，即默克尔细胞多瘤病毒（MCV）是一个致病因素的基础上，大多数情况下，一种高度致命形式的皮肤癌称为默克尔细胞癌。为了探讨多瘤病毒普遍感染健康人皮肤的可能性，我们开发了一种改进的滚环扩增（RCA）技术，从皮肤拭子标本中分离环状DNA病毒基因组。从14/35（40%）健康成人中回收了完整的MCV基因组，为该多瘤病毒物种提供了第一个全长的、显然是野生型的克隆基因组。RCA分析还揭示了两种以前未知的多瘤病毒物种的存在，我们将其命名为人类多瘤病毒-6（HPyV 6）和HPyV 7。生物化学实验表明，多瘤病毒DNA以组装的病毒体的形式从皮肤脱落。一项初步血清学研究表明，三种皮肤嗜性多瘤病毒的感染或合并感染非常常见。因此，至少三种多瘤病毒属是人类皮肤微生物组的组成部分。
Mounting evidence supports the concept that Merkel cell polyomavirus (MCV) is a causal factor underlying most cases of a highly lethal form of skin cancer known as Merkel cell carcinoma. To explore the possibility that polyomaviruses commonly infect healthy human skin, we developed an improved rolling circle amplification (RCA) technique to isolate circular DNA viral genomes from skin swab specimens. Complete MCV genomes were recovered from 14/35 (40%) healthy adults, providing the first full-length, apparently wild-type cloned genomes for this polyomavirus species. RCA analysis also revealed the existence of two previously unknown polyomavirus species that we name human polyomavirus-6 (HPyV6) and HPyV7. Biochemical experiments show that polyomavirus DNA is shed from the skin in the form of assembled virions. A pilot serological study indicates that infection or co-infection with the three skin-tropic polyomaviruses is very common. Thus, at least three polyomavirus species are constituents of the human skin microbiome.
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