Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.
Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.
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DOI：
10.1002/dvdy.21925
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发表时间：
2009-05
影响因子：
2.5
通讯作者：
Koopman, Peter
中科院分区：
文献类型：
--
作者：
Combes, Alexander N.;Lesieur, Emmanuelle;Harley, Vincent R.;Sinclair, Andrew H.;Little, Melissa H.;Wilhelm, Dagmar;Koopman, Peter
睾丸索是产生和输出精子必不可少的特殊管道，但指导其形成的机制以及其位置，大小，形状和数量的调节仍不清楚。在这里，我们使用一种新的荧光为基础的三维建模方法，以显示索最初形成的不规则的细胞簇，随后重塑，形成规则的平行循环，连接在中肾侧的扁平丛的网络。脐带数量和结构的变化表明，虽然脐带排列和直径变得相对一致，但脐带规格不是定型的，这意味着补偿性生长机制。通常观察到分支、融合和内化的索。我们的结论是，睾丸索的管状结构出现通过一种新的形式的形态发生的合并，分区和重塑。我们所描述的方法适用于研究小鼠模型中睾丸索发育的缺陷，更广泛地说，研究其他组织的形态发生。
Testis cords are specialised tubes essential for generation and export of sperm, yet the mechanisms directing their formation, and the regulation of their position, size, shape and number, remain unclear. Here, we use a novel fluorescence-based three-dimensional modelling approach to show that cords initially form as a network of irregular cell clusters that are subsequently remodelled to form regular parallel loops, joined by a flattened plexus at the mesonephric side. Variation in cord number and structure demonstrates that cord specification is not stereotypic, although cord alignment and diameter becomes relatively consistent, implicating compensatory growth mechanisms. Branched, fused and internalised cords were commonly observed. We conclude that the tubule-like structure of testis cords arise through a novel form of morphogenesis consisting of coalescence, partitioning, and remodelling. The methods we describe are applicable to investigating defects in testis cord development in mouse models, and more broadly, studying morphogenesis of other tissues.
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影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
DOI：
10.1196/annals.1411.015
发表时间：
2007-01-01
期刊：
TESTICULAR CHROMOSOME STRUCTURE AND GENE EXPRESSION
影响因子：
--
作者：
Gassei, Kathrin;Schlatt, Stefan
通讯作者：
Schlatt, Stefan
影响因子：
--
作者：
PARANKO, J
通讯作者：
PARANKO, J
DOI：
10.1111/j.1749-6632.1984.tb38302.x
发表时间：
1984-01-01
影响因子：
5.2
作者：
PELLINIEMI, LJ;PARANKO, J;LAKKALAPARANKO, T
通讯作者：
LAKKALAPARANKO, T
影响因子：
2.7
作者：
Wilhelm, D;Martinson, F;Koopman, P
通讯作者：
Koopman, P