Differentiation of adult stem cells into smooth muscle for vascular tissue engineering.
Differentiation of adult stem cells into smooth muscle for vascular tissue engineering.
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DOI：
10.1016/j.jss.2009.08.001
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发表时间：
2011-06-15
期刊：
影响因子：
--
通讯作者：
DiMuzio PJ
中科院分区：
文献类型：
--
作者：
Harris LJ;Abdollahi H;Zhang P;McIlhenny S;Tulenko TN;DiMuzio PJ
在此，我们评估了脂肪干细胞(ASC)分化为平滑肌细胞(SMC)的潜力，以及它们在组织工程化血管移植中的应用潜力。我们从血管手术患者脐周脂肪组织中分离出(CD13+29+90+)ASC，并在含有血管紧张素II(AngII)、鞘氨醇磷脂酰胆碱(SPC)或转化生长因子-β1(TGF1)的培养液中培养3周。通过RT-PCR、qPCR和Western印迹检测早期(Calponin，caldesmon)和晚期(Myosin Heavy Chain，MHC)SMC标志物的表达，以及2)胶原凝胶上培养的SMC收缩。将分化的ASCs接种到生物反应器内的血管移植物(去细胞的大隐静脉)上，用共聚焦显微镜测定细胞贴壁。分化前，ASC三种分子标志物均低水平表达。在每种分化介质中培养后，所有细胞系中Calponin、Caldesmon和MHC上调的程度是不同的。在胶原凝胶上种植后，在SPC中分化的ASC和转化生长因子β-1表现出收缩特性，类似于平滑肌细胞对照。分化的干细胞在血管移植物上黏附和增殖。这些结果提示：1)分化后的人脂肪干细胞表达不同的SMC分子标志物，2)经SPC和转化生长因子β-1诱导分化后表现为收缩表型，3)在血管移植物支架上增殖。因此，ASCs在构建自体动脉方面具有潜在的应用价值。
Herein we evaluate the potential of adipose-derived stem cells (ASC) to differentiate into smooth muscle cells (SMC) and their potential for use in a tissue-engineered vascular graft. We isolated ASC (CD13+29+90+) from the peri-umbilical adipose tissue of patients undergoing vascular surgery, and cultured them in media containing angiotensin II (AngII), sphingosylphosphorylcholine (SPC) or transforming growth factor-beta 1 (TGFβ1) for up to three weeks. SMC differentiation was assessed by: 1) expression of early (calponin, caldesmon) and late (myosin heavy chain, MHC) SMC markers by RT-PCR, qPCR and Western blot, and 2) contraction upon plating on collagen gel. Differentiated ASCs were seeded onto a vascular graft (decellularized saphenous vein) within a bioreactor, and cell attachment was determined using confocal microscopy. Prior to differentiation, ASC expressed low levels of all three molecular markers. After culture in each differentiating medium, the extent of up-regulation of calponin, caldesmon and MHC was variable across all cell lines. After seeding onto collagen gel, ASC differentiated in SPC and TGFβ1 exhibit contractile properties, similar to smooth muscle cell controls. Differentiated stem cells adhered and proliferated on the vascular graft. These data suggest that human adipose-derived stem cells: 1) exhibit variable expression of SMC molecular markers after differentiation, 2) exhibit a contractile phenotype after differentiation with SPC and TGFβ1, and 3) proliferate on a vascular graft scaffold. Thus, ASCs are potentially useful in the construction of autologous arteries.
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影响因子：
4.8
作者：
Gong, Zhaodi;Niklason, Laura E.
通讯作者：
Niklason, Laura E.
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
14
作者：
Wu, Hsi-Chin;Wang, Tzu-Wei;Lin, Feng-Huei
通讯作者：
Lin, Feng-Huei
影响因子：
5.2
作者：
Katz, AJ;Tholpady, A;Ogle, RC
通讯作者：
Ogle, RC