Addition of algicidal bacterium CZBC1 and molasses to inhibit cyanobacteria and improve microbial communities, water quality and shrimp performance in culture systems
Addition of algicidal bacterium CZBC1 and molasses to inhibit cyanobacteria and improve microbial communities, water quality and shrimp performance in culture systems
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添加杀藻细菌 CZBC1 和糖蜜以抑制蓝藻并改善养殖系统中的微生物群落、水质和对虾性能
DOI：
10.1016/j.aquaculture.2018.12.063
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发表时间：
2019-03
期刊：
影响因子：
4.5
通讯作者：
Yucheng Cao
中科院分区：
文献类型：
--
作者：
Wujie Xu;Yu Xu;Xiaoshuai Huang;Xiaojuan Hu;Yunna Xu;Haochang Su;Zhuojia Li;Keng Yang;Guoliang Wen;Yucheng Cao
近年来，蓝藻水华在水产养殖过程中发生的频率越来越高。它们可能对水产养殖系统造成破坏，并带来巨大的经济损失。因此，有必要开发有效的和生态友好的方法来控制蓝藻水华。通过为期56天的田间试验，研究了溶藻细菌CZBC 1（B组）及其与糖蜜（BC组）联合使用对凡纳滨对虾养殖系统蓝藻水华的防治效果。在实验期间，B组和BC组的颤藻和微藻密度均逐渐下降，实验结束时颤藻占微藻的百分比均显著下降至<4%。与对照组相比，B组和BC组的细菌总数和可培养异养菌数增加较快。16 SrRNA高通量测序结果显示，培养7 d后，B组和BC组中蓝藻门和颤藻纲的相对丰度下降到很低的水平，BC组中变形菌门和拟杆菌门的相对丰度较高。各试验组的细菌丰富度（Chao 1和ACE）均逐渐增加，而糖蜜的添加对细菌群落的α多样性（Shannon和Simpson指数）有一定的影响。与对照组相比，B组和BC组的氨氮和亚硝酸盐氮峰值浓度显著降低，糖蜜显著促进了总悬浮物浓度的升高。因此，B组和BC组的养殖对虾的成活率、最终体重、生长率和饲料转化率均有所提高。值得注意的是，当CZBC 1和糖蜜组合用于蓝藻控制时，可以发现协同效应。本研究结果为水产养殖系统中蓝藻的控制提供了一种很有前途的实用方法。
In recent years, cyanobacterial blooms occur with increasing frequency during the process of aquaculture. They could cause damage on aquaculture systems and bring huge economic losses. It is necessary to develop effective and ecofriendly methods to control cyanobacterial blooms. In this study, a 56-day field experiment was conducted to assess the effects of algicidal bacterium CZBC1 (group B) and its combined use with molasses (group BC) against a cyanobacterial bloom inLitopenaeus vannameiculture systems. During the experimental period, the densities ofOscillatoriaand microalgae decreased gradually in groups B and BC, and both percentages ofOscillatoriain microalgae decreased significantly to <4% at the end of the experiment. Meanwhile, the densities of total bacteria and cultivable heterotrophic bacteria increased faster in groups B and BC compared with the control group. The result from 16S rRNA high-throughput sequencing showed that the relative abundance of the Cyanobacteria phylum and the Oscillatoriophycideae class in groups B and BC decreased to very low levels, and the phyla of Proteobacteria and Bacteroidetes were more abundant in group BC after 7 days. The bacterial richness (Chao1 and ACE) increased gradually in all experimental groups, while the addition of molasses appeared to have some effects on α-diversity (Shannon and Simpson indices) of bacterial community. Furthermore, the peak concentrations of total ammonia nitrogen and nitrite-nitrogen were found to be significantly lower in groups B and BC compared with the control group, and molasses significantly promoted the increase of total suspended solids concentration. Consequently, survival, final weight, growth rate and feed conversion rate of cultured shrimp were improved in groups B and BC. Notably, a synergistic effect could be found when CZBC1 and molasses were used in combination for cyanobacteria control. The results of this study indicate a promising practical method for cyanobacteria control in aquaculture systems.
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