Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis.
Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis.
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DOI：
10.1038/nsmb.2735
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发表时间：
2014-01
影响因子：
16.8
通讯作者：
Jacobsen SE
中科院分区：
文献类型：
--
作者：
Stroud H;Do T;Du J;Zhong X;Feng S;Johnson L;Patel DJ;Jacobsen SE
DNA甲基化发生在CG和非CG序列环境中。非CG甲基化在植物中大量存在，并由染色质甲基化酶（CMT）和结构域重排甲基转移酶（DRM）蛋白介导;然而，其作用仍然知之甚少。在这里，我们的特点在拟南芥中的非CG甲基化的作用。我们发现，一个不好的特点甲基转移酶，CMT 2，是一个功能性的甲基转移酶在体外和体内。CMT 2优先结合组蛋白H3赖氨酸9（H3 K9）二甲基化并甲基化受H3 K9甲基化调节的非CG胞嘧啶。我们揭示了每个非CG甲基转移酶在DNA甲基化模式和调节转录中的贡献和冗余。我们还证明了小RNA积累和H3 K9甲基化模式对非CG甲基化的广泛依赖性，表明这些表观遗传因素之间存在自我增强机制。结果表明，非CG甲基化模式在形成组蛋白修饰和小的非编码RNA景观中至关重要。
DNA methylation occurs in CG and non-CG sequence contexts. Non-CG methylation is abundant in plants, and is mediated by CHROMOMETHYLASE (CMT) and DOMAINS REARRANGED METHYLTRANSFERASE (DRM) proteins; however its roles remain poorly understood. Here we characterize the roles of non-CG methylation in Arabidopsis thaliana. We show that a poorly characterized methyltransferase, CMT2, is a functional methyltransferase in vitro and in vivo. CMT2 preferentially binds histone H3 lysine 9 (H3K9) dimethylation and methylates non-CG cytosines that are regulated by H3K9 methylation. We revealed the contributions and redundancies between each non-CG methyltransferase in DNA methylation patterning and in regulating transcription. We also demonstrate extensive dependencies of small RNA accumulation and H3K9 methylation patterning on non-CG methylation, suggesting self-reinforcing mechanisms between these epigenetic factors. The results suggest that non-CG methylation patterns are critical in shaping the histone modification and small non-coding RNA landscapes.
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影响因子：
11.4
作者：
Roudier, Francois;Ahmed, Ikhlak;Berard, Caroline;Sarazin, Alexis;Mary-Huard, Tristan;Cortijo, Sandra;Bouyer, Daniel;Caillieux, Erwann;Duvernois-Berthet, Evelyne;Al-Shikhley, Liza;Giraut, Laurene;Despres, Barbara;Drevensek, Stephanie;Barneche, Fredy;Derozier, Sandra;Brunaud, Veronique;Aubourg, Sebastien;Schnittger, Arp;Bowler, Chris;Martin-Magniette, Marie-Laure;Robin, Stephane;Caboche, Michel;Colot, Vincent
通讯作者：
Colot, Vincent
影响因子：
11.4
作者：
Soppe, WJJ;Jasencakova, Z;Fransz, PF
通讯作者：
Fransz, PF
DOI：
10.1007/978-1-61779-089-8_16
发表时间：
2011-01-01
期刊：
HIGH-THROUGHPUT NEXT GENERATION SEQUENCING: METHODS AND APPLICATION
影响因子：
--
作者：
Feng, Suhua;Rubbi, Liudmilla;Pellegrini, Matteo
通讯作者：
Pellegrini, Matteo
影响因子：
4.1
作者：
McCue AD;Nuthikattu S;Slotkin RK
通讯作者：
Slotkin RK
影响因子：
64.8
作者：
Jackson, JP;Lindroth, AM;Jacobsen, SE
通讯作者：
Jacobsen, SE