Integrative epigenomic mapping defines four main chromatin states in Arabidopsis.
Integrative epigenomic mapping defines four main chromatin states in Arabidopsis.
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DOI：
10.1038/emboj.2011.103
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发表时间：
2011-05-18
期刊：
影响因子：
11.4
通讯作者：
Colot, Vincent
中科院分区：
文献类型：
--
作者：
Roudier, Francois;Ahmed, Ikhlak;Berard, Caroline;Sarazin, Alexis;Mary-Huard, Tristan;Cortijo, Sandra;Bouyer, Daniel;Caillieux, Erwann;Duvernois-Berthet, Evelyne;Al-Shikhley, Liza;Giraut, Laurene;Despres, Barbara;Drevensek, Stephanie;Barneche, Fredy;Derozier, Sandra;Brunaud, Veronique;Aubourg, Sebastien;Schnittger, Arp;Bowler, Chris;Martin-Magniette, Marie-Laure;Robin, Stephane;Caboche, Michel;Colot, Vincent
在真核生物中，组蛋白的翻译后修饰和DNA甲基化是染色质水平控制基因组活性的重要组成部分。然而，控制染色质标记沿基因组的组合关联的原理仍然知之甚少。在这里，我们已经在模式植物拟南芥中生成了八种组蛋白修饰(H3K4me2和3、H3K27me1和2、H3K36me3、H3K56ac、H4K20me1和H2Bub)的表观基因组图谱，并将这些图谱与在相同条件下产生的其他图谱相结合，分别针对H3K9me2、H3K9me3、H3K27me3和DNA甲基化。综合分析表明，这12个染色质标记总共覆盖了∼基因组的90%，以非常有限的组合出现在任何给定的位置。此外，我们还发现，这12个标记沿基因组序列的分布定义了四种主要的染色质状态，它们优先索引活性基因、抑制基因、沉默重复元件和基因间隔区。由于拟南芥基因组的紧凑性质，这四个索引状态通常被翻译成相互穿插的短染色质结构域。拟南芥表观基因组的这第一个组合观点指出了后生动物中简单的组织原理，并为进一步研究植物中基于染色质的调控机制提供了一个框架。
Post-translational modification of histones and DNA methylation are important components of chromatin-level control of genome activity in eukaryotes. However, principles governing the combinatorial association of chromatin marks along the genome remain poorly understood. Here, we have generated epigenomic maps for eight histone modifications (H3K4me2 and 3, H3K27me1 and 2, H3K36me3, H3K56ac, H4K20me1 and H2Bub) in the model plant Arabidopsis and we have combined these maps with others, produced under identical conditions, for H3K9me2, H3K9me3, H3K27me3 and DNA methylation. Integrative analysis indicates that these 12 chromatin marks, which collectively cover ∼90% of the genome, are present at any given position in a very limited number of combinations. Moreover, we show that the distribution of the 12 marks along the genomic sequence defines four main chromatin states, which preferentially index active genes, repressed genes, silent repeat elements and intergenic regions. Given the compact nature of the Arabidopsis genome, these four indexing states typically translate into short chromatin domains interspersed with each other. This first combinatorial view of the Arabidopsis epigenome points to simple principles of organization as in metazoans and provides a framework for further studies of chromatin-based regulatory mechanisms in plants.
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影响因子：
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