Association between hypertension and circulating vascular-related microRNAs.
Association between hypertension and circulating vascular-related microRNAs.
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DOI：
10.1038/s41371-018-0061-2
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发表时间：
2018-06
影响因子：
2.7
通讯作者：
DeSouza CA
中科院分区：
文献类型：
--
作者：
Hijmans JG;Diehl KJ;Bammert TD;Kavlich PJ;Lincenberg GM;Greiner JJ;Stauffer BL;DeSouza CA
microRNAs （miRNAs）在调节炎症、血管健康以及心血管疾病中发挥着关键作用。具体来说，miR-17、miR-21、miR-34a、miR-92a、miR-126、miR-145、miR-146a和miR-150的循环表达改变与心血管疾病的发病和进展有关。本研究的目的是确定这些血管相关mirna的循环谱是否因高血压而中断。研究了30名久坐不动的中年人：15名血压正常（10米/5F；年龄：56±1岁；血压：114/71±2/1毫米汞柱），15名高血压（10米/5F； 56±2岁；140/87±2/2毫米汞柱）。所有受试者均非肥胖且无其他心脏代谢紊乱。使用标准RT-PCR技术和感兴趣的miRNA引物测定血浆中的循环miRNA。将表达归一化为外源秀丽隐杆线虫miR-39，并报告为任意单位（AU）的相对表达。miR-34a循环表达（9.18±0.94 vs 5.33±0.91 AU）升高（~170%，P<0.01），而miR-21（1.32±0.25 vs 2.50±0.29 AU）、miR-126（0.85±0.10 vs 1.74±0.27 AU）和miR-146a（1.50±0.20 vs 3.10±0.50 AU）的表达明显降低（分别为~50%、~55%和~55%，P<0.05）。此外，miR-34a、miR-21和miR-126的循环水平与收缩压显著相关（r=0.48, r= - 0.38; r= - 0.48）；而miR-146a与收缩压（r= - 0.58）和舒张压（r= - 0.55）均显著相关。各组循环miR-17、miR-92a、miR-145和miR-150无显著差异。总之，这些结果表明，独立于其他心脏代谢危险因素的高血压，对与心血管疾病风险和发展相关的一组血管相关mirna的循环谱产生不利影响。
microRNAs (miRNAs) play a key role in regulating inflammation, vascular health and in-turn, cardiovascular disease. Specifically, altered circulating expression of miR-17, miR-21, miR-34a, miR-92a, miR-126, miR-145, miR-146a and miR-150 has been linked with the pathogenesis and progression of cardiovascular disease. The aim of this study was to determine whether the circulating profile of these vascular-related miRNAs is disrupted with hypertension. Thirty sedentary, middle-aged adults were studied: 15 normotensive (10M/5F; age: 56±1 yr; BP: 114/71±2/1 mmHg) and 15 hypertensive (10M/5F; 56±2 yr; 140/87±2/2 mmHg). All subjects were non-obese and free of other cardiometabolic disorders. Circulating miRNAs were determined in plasma using standard RT-PCR techniques with miRNA primers of interest. Expression was normalized to exogenous C. elegans miR-39 and reported as relative expression in arbitrary units (AU). Circulating expression of miR-34a (9.18±0.94 vs 5.33±0.91 AU) was higher (~170%; P<0.01) whereas the expression of miR-21 (1.32±0.25 vs 2.50±0.29 AU), miR-126 (0.85±0.10 vs 1.74±0.27 AU) and miR-146a (1.50±0.20 vs 3.10±0.50 AU) were markedly lower (~50%, ~55% and ~55% respectively; P<0.05) in the hypertensive vs normotensive groups. Moreover, circulating levels of miR-34a, miR-21 and miR-126 were significantly related to systolic blood pressure (r=0.48, r=−0.38; r=−0.48); whereas, miR-146a was significantly related to both systolic (r=−0.58) and diastolic (r=−0.55) blood pressure. There were no significant group differences in circulating miR-17, miR-92a, miR-145 and miR-150. In summary, these results suggest that hypertension, independent of other cardiometabolic risk factors, adversely affects the circulating profile of a subset of vascular-related miRNAs that have been link to CVD risk and development.
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DOI：
10.1016/j.bbrc.2010.02.045
发表时间：
2010-03-19
影响因子：
3.1
作者：
Weber, Martina;Baker, Meredith B.;Moore, Jeffrey P.;Searles, Charles D.
通讯作者：
Searles, Charles D.
影响因子：
13.6
作者：
Andreou I;Sun X;Stone PH;Edelman ER;Feinberg MW
通讯作者：
Feinberg MW
影响因子：
12.8
作者：
Han, Hui;Qu, Guangjin;Han, Chenghua;Wang, Yuhong;Sun, Tingting;Li, Fengqing;Wang, Junxiao;Luo, Shanshun
通讯作者：
Luo, Shanshun
影响因子：
3.5
作者：
Chen, Lingqiang;Wang, Jing;Du, Kaili
通讯作者：
Du, Kaili
影响因子：
20.1
作者：
Rahimi, Kazem;Emdin, Connor A.;MacMahon, Stephen
通讯作者：
MacMahon, Stephen