6-sulfatoxymelatonin collected from infant diapers: feasibility and implications for urinary biochemical markers.
6-sulfatoxymelatonin collected from infant diapers: feasibility and implications for urinary biochemical markers.
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DOI：
10.1177/1099800409337330
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发表时间：
2010-01
影响因子：
2.5
通讯作者：
Thomas KA
中科院分区：
文献类型：
--
作者：
Thomas KA
本研究的目的是评估使用尿布垫收集家庭婴儿尿液样本的可行性和可接受性，并测试尿布垫提取6-硫酸氧褪黑激素和肌酐的准确性，用于校正尿量的测定结果。为了评估可行性和可接受性，使用插入尿布中的棉垫收集了20名婴儿24小时的尿液样本。尿垫提取的准确性进行了评估，在实验室设置使用尿液样本收集11名成年志愿者，并采用酶免疫吸附试验（EIA）进行测定。将尿液样本分开，一个等分试样在未提取的情况下进行分析，一个等分试样滴入尿布垫中，提取并进行分析。妈妈们发现收集尿布垫是可以接受的，而且很容易完成。在家庭环境中获得的144个婴儿尿液样本中，59%可用于检测目的，其余的要么被粪便污染，要么体积不足。虽然来自尿布垫提取和未提取样品的肌酸酐值高度相关（R2 = 0.947），但肌酸酐校正的6-硫酸氧褪黑激素的肌酸酐值不相关（R2 = 0.216）。尿布垫收集程序改变6-硫酸氧褪黑激素值。尿生化物质的测量和统计分析的意义进行了讨论。
The purpose of this study was to assess feasibility and acceptability of using a diaper pad for collection of in-home infant urinary samples and to test the accuracy of diaper pad extraction for 6-sulfatoxymelatonin and creatinine, which was used to correct assay results for urinary volume. To assess feasibility and acceptability, urine samples from 20 infants were collected over a 24-hour day using a cotton pad inserted in the diaper. The accuracy of diaper pad extraction was evaluated in the laboratory setting using urine samples collected from 11 adult volunteers and assayed using enzyme immunosorbent assay (EIA). Urine samples were divided, one aliquot was assayed without extraction and one aliquot was instilled into a diaper pad, extracted, and assayed. Mothers found diaper pad collection acceptable and easy to perform. Of 144 infant urinary samples obtained in the home environment, 59% were usable for assay purposes, the remaining either were contaminated with stool or were of insufficient volume. While creatinine values from diaper pad extracted and non-extracted samples were highly correlated (R2 = 0.947) those of creatinine corrected 6-sulfatoxymelatonin were not (R2 = 0.216). Diaper pad collection procedures altered 6-sulfatoxymelatonin values. Implications for measurement of urinary biochemical substances and statistical analysis are discussed.
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DOI：
10.1038/sj.jea.7500334
发表时间：
2004-09-01
期刊：
JOURNAL OF EXPOSURE ANALYSIS AND ENVIRONMENTAL EPIDEMIOLOGY
影响因子：
--
作者：
Hu, Y;Beach, J;Gardner, M
通讯作者：
Gardner, M
影响因子：
3.2
作者：
MATTHEWS, CD;GUERIN, MV;WANG, X
通讯作者：
WANG, X
影响因子：
5.2
作者：
Rao, S;Bhatt, J;Macfarlane, P
通讯作者：
Macfarlane, P
影响因子：
3.6
作者：
Heckmann, M;Hartmann, MF;Wudy, SA
通讯作者：
Wudy, SA
影响因子：
6.6
作者：
Dorey, Corine M.;Zimmermann, Michael B.
通讯作者：
Zimmermann, Michael B.