The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid.
The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid.
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猪链球菌转录景观揭示了猪血和脑脊液中的适应机制。
DOI：
10.1261/rna.041822.113
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Wu Z;Wu C;Shao J;Zhu Z;Wang W;Zhang W;Tang M;Pei N;Fan H;Li J;Yao H;Gu H;Xu X;Lu C
在这项差异RNA-seq研究中，Wu等人鉴定了猪病原体猪链球菌（Streptococcus suis）表达的29种小RNA。作者接着指出S.缺乏这些小RNA的猪菌株在斑马鱼感染模型中被减毒，或者对猪血杀死更敏感。因此，这项研究提供了进一步的证据，表明小的调控RNA在细菌毒力中发挥作用。猪链球菌（Streptococcus suis，SS）是一种重要的猪源性病原菌，也是一种人畜共患病病原。SS感染可导致宿主败血症或脑膜炎。然而，很少有人知道的基因，有助于毒力过程和宿主血液或脑脊液（CSF）中的生存。小RNA（sRNA）已成为几种细菌毒力的关键调节因子，但在SS中尚未研究。在这里，使用差异RNA测序方法和RNA从SS菌株P1/7生长在丰富的培养基，猪血，或CSF，我们提出了SS全基因组图谱的793个转录起始位点和370个操纵子。除了确定29个sRNA，我们表明，5个sRNA缺失突变体在斑马鱼感染模型中减弱SS毒力。同源性搜索显示，10个sRNAs被预测存在于其他致病性链球菌物种。与野生型菌株P1/7相比，sRNArss 03、rss 05和rss 06缺失突变株对猪血的杀伤作用显著增强。rss 06可能通过间接激活编码锌结合脂蛋白的毒力基因SSU 0308的表达而促进SS毒力。在血液中，参与荚膜多糖（CPS）合成和破坏宿主防御的基因上调。相反，在CSF中，CPS合成基因下调。我们的研究是第一次分析参与毒力的SS sRNA，既提高了我们对SS发病机制的理解，又增加了已知在细菌毒力中起决定性作用的sRNA的数量。
In this differential RNA-seq study, Wu et al. identified 29 small RNAs expressed by the pig pathogen Streptococcus suis. The authors then went on to show that S. suis strains lacking some of these small RNAs were attenuated in a zebrafish infection model or were more sensitive to killing by pig blood. The study thus provides further evidence that small, regulatory RNAs play roles in bacterial virulence. Streptococcus suis (SS) is an important pathogen of pigs, and it is also recognized as a zoonotic agent for humans. SS infection may result in septicemia or meningitis in the host. However, little is known about genes that contribute to the virulence process and survival within host blood or cerebrospinal fluid (CSF). Small RNAs (sRNA) have emerged as key regulators of virulence in several bacteria, but they have not been investigated in SS. Here, using a differential RNA-sequencing approach and RNAs from SS strain P1/7 grown in rich medium, pig blood, or CSF, we present the SS genome-wide map of 793 transcriptional start sites and 370 operons. In addition to identifying 29 sRNAs, we show that five sRNA deletion mutants attenuate SS virulence in a zebrafish infection model. Homology searches revealed that 10 sRNAs were predicted to be present in other pathogenic Streptococcus species. Compared with wild-type strain P1/7, sRNAs rss03, rss05, and rss06 deletion mutants were significantly more sensitive to killing by pig blood. It is possible that rss06 contributes to SS virulence by indirectly activating expression of SSU0308, a virulence gene encoding a zinc-binding lipoprotein. In blood, genes involved in the synthesis of capsular polysaccharide (CPS) and subversion of host defenses were up-regulated. In contrast, in CSF, genes for CPS synthesis were down-regulated. Our study is the first analysis of SS sRNAs involved in virulence and has both improved our understanding of SS pathogenesis and increased the number of sRNAs known to play definitive roles in bacterial virulence.
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影响因子：
6.7
作者：
Echenique-Rivera H;Muzzi A;Del Tordello E;Seib KL;Francois P;Rappuoli R;Pizza M;Serruto D
通讯作者：
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影响因子：
6.7
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3.1
作者：
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
3.3
作者：
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通讯作者：
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