Long-time treatment by low-dose N-acetyl-L-cysteine enhances proinflammatory cytokine expressions in LPS-stimulated macrophages.
Long-time treatment by low-dose N-acetyl-L-cysteine enhances proinflammatory cytokine expressions in LPS-stimulated macrophages.
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DOI：
10.1371/journal.pone.0087229
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Matsuguchi T
中科院分区：
文献类型：
--
作者：
Ohnishi T;Bandow K;Kakimoto K;Kusuyama J;Matsuguchi T
n -乙酰- l-半胱氨酸被认为是一种活性氧清除剂，并被用于临床应用。先前的报道表明，高剂量n -乙酰- l-半胱氨酸治疗可抑制活化巨噬细胞中促炎细胞因子的表达。在这里，我们发现长时间低浓度的n -乙酰- l-半胱氨酸处理增加了细胞外信号调节激酶1/2和AKT的磷酸化，这对于在脂多糖刺激的RAW264.7细胞中诱导包括白细胞介素1β和白细胞介素6在内的促炎细胞因子至关重要。此外，长时间的n -乙酰- l-半胱氨酸处理降低了蛋白磷酸酶、蛋白磷酸酶- 2a催化亚基和双特异性磷酸酶1的表达。另一方面，我们发现短时间低剂量n -乙酰- l-半胱氨酸治疗可增加p53表达，抑制促炎细胞因子的表达。这些观察结果表明，长时间低剂量n -乙酰- l-半胱氨酸治疗通过增强激酶磷酸化增加促炎细胞因子的表达。
N-acetyl-L-cysteine is known to act as a reactive oxygen species scavenger and used in clinical applications. Previous reports have shown that high-dose N-acetyl-L-cysteine treatment inhibits the expression of proinflammatory cytokines in activated macrophages. Here, we have found that long-time N-acetyl-L-cysteine treatment at low-concentration increases phosphorylation of extracellular signal-regulated kinase 1/2 and AKT, which are essential for the induction of proinflammatory cytokines including interleukin 1β and interleukin 6 in lipopolysaccharide-stimulated RAW264.7 cells. Furthermore, long-time N-acetyl-L-cysteine treatment decreases expressions of protein phosphatases, catalytic subunit of protein phosphatase-2A and dual specificity phosphatase 1. On the other hand, we have found that short-time N-acetyl-L-cysteine treatment at low dose increases p53 expression, which inhibits expressions of proinflammatory cytokines. These observations suggest that long-time low-dose N-acetyl-L-cysteine treatment increases expressions of proinflammatory cytokines through enhancement of kinase phosphorylation.
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影响因子：
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作者：
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DOI：
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