Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor.
Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor.
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DOI：
10.1242/jcs.207779
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发表时间：
2018-03-26
影响因子：
4
通讯作者：
Pichaud F
中科院分区：
文献类型：
--
作者：
Walther RF;Burki M;Pinal N;Rogerson C;Pichaud F
在果蝇上皮细胞中，顶端的Bazooka蛋白（果蝇Par3蛋白）的排除决定了粘附带（zonula adherens， ZA）的位置，ZA区分了顶端和外侧膜，并允许细胞组装成薄片。在这里，我们发现小的GTPase Rap1，它的效应物Canoe （Cno）和Cdc42效应激酶Mushroom bodies tiny (Mbt)，通过偶联稳定粘附连接（AJ）蛋白E-Cadherin和ZA上的Bazooka保留来调节上皮形态发生。此外，我们的研究结果表明，Rap1、Cno和Mbt在ZA的定位是相互依赖的，表明它们在ZA形态发生过程中的功能是相互联系的。在这种情况下，我们发现Rap1- gef Dizzy在ZA富集，我们的结果表明它在ZA形态发生过程中促进Rap1的活性。综上所述，我们认为Dizzy、Rap1和Cno通路以及Mbt共同调控Bazooka与AJ材料之间的界面，促进ZA的形态发生。摘要：在果蝇上皮细胞形态发生过程中，趋同通路调节粘附体连接的稳定性和Bazooka的保留，促进粘附体的成熟。
In Drosophila epithelial cells, apical exclusion of Bazooka (the Drosophila Par3 protein) defines the position of the zonula adherens (ZA), which demarcates the apical and lateral membrane and allows cells to assemble into sheets. Here, we show that the small GTPase Rap1, its effector Canoe (Cno) and the Cdc42 effector kinase Mushroom bodies tiny (Mbt), converge in regulating epithelial morphogenesis by coupling stabilization of the adherens junction (AJ) protein E-Cadherin and Bazooka retention at the ZA. Furthermore, our results show that the localization of Rap1, Cno and Mbt at the ZA is interdependent, indicating that their functions during ZA morphogenesis are interlinked. In this context, we find the Rap1-GEF Dizzy is enriched at the ZA and our results suggest that it promotes Rap1 activity during ZA morphogenesis. Altogether, we propose the Dizzy, Rap1 and Cno pathway and Mbt converge in regulating the interface between Bazooka and AJ material to promote ZA morphogenesis. Summary: Converging pathways regulate adherens junction stability and retention of Bazooka to promote zonula adherens maturation during epithelial cell morphogenesis in Drosophila.
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影响因子：
3.7
作者：
Law, Sheran H. W.;Sargent, Thomas D.
通讯作者：
Sargent, Thomas D.
影响因子：
1.5
作者：
Hayashi, S;Ito, K;Goto, S
通讯作者：
Goto, S
影响因子：
64.5
作者：
Freeman, M
通讯作者：
Freeman, M
影响因子：
64.5
作者：
Morais-de-Sá E;Mirouse V;St Johnston D
通讯作者：
St Johnston D
DOI：
10.1083/jcb.201006029
发表时间：
2010-09-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Krahn MP;Bückers J;Kastrup L;Wodarz A
通讯作者：
Wodarz A