aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
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DOI：
10.1016/j.cell.2010.02.040
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发表时间：
2010-04-30
期刊：
影响因子：
64.5
通讯作者：
St Johnston D
中科院分区：
文献类型：
--
作者：
Morais-de-Sá E;Mirouse V;St Johnston D
Bazooka（PAR-3）、PAR-6和aPKC形成复合物，在许多细胞类型的极化中起关键作用。然而，在上皮细胞中，Bazooka定位于PAR-6和aPKC下方的顶端/外侧交界处。在这里，我们表明，Baz被排除在顶端aPKC结构域在上皮细胞的aPKC磷酸化，这破坏了Baz/aPKC的相互作用。从复合物中去除Baz是上皮特异性的，因为它还需要阻止Baz/PAR-6相互作用的Crumbs复合物。在不存在Baz的Crumbs或aPKC磷酸化的情况下，错误定位的Baz在顶端募集粘附连接组分，导致顶端结构域的丧失和侧向结构域的扩张。因此，Crumbs和aPKC对Baz的顶端排斥定义了顶端/外侧边界。虽然Baz在非上皮细胞中作为aPKC靶向和特异性因子，但我们的研究结果表明，它在上皮细胞中定位粘附连接方面具有互补功能。CaPKC磷酸化Bazooka以阻止其与aPKC的结合CaCrumbs在上皮细胞中竞争磷酸化Baz与PAR-6的结合CaCaCaCrumbs在上皮细胞中竞争磷酸化Baz与PAR-6的结合CaCaCrumbs在上皮细胞中竞争磷酸化Baz与PAR-6的结合CaCrumbs在上皮细胞中竞争磷酸化Baz与Baz的结合CaCrumbs在
Bazooka (PAR-3), PAR-6, and aPKC form a complex that plays a key role in the polarization of many cell types. In epithelial cells, however, Bazooka localizes below PAR-6 and aPKC at the apical/lateral junction. Here, we show that Baz is excluded from the apical aPKC domain in epithelia by aPKC phosphorylation, which disrupts the Baz/aPKC interaction. Removal of Baz from the complex is epithelial-specific because it also requires the Crumbs complex, which prevents the Baz/PAR-6 interaction. In the absence of Crumbs or aPKC phosphorylation of Baz, mislocalized Baz recruits adherens junction components apically, leading to a loss of the apical domain and an expansion of lateral. Thus, apical exclusion of Baz by Crumbs and aPKC defines the apical/lateral border. Although Baz acts as an aPKC targeting and specificity factor in nonepithelial cells, our results reveal that it performs a complementary function in positioning the adherens junction in epithelia. ► aPKC phosphorylates Bazooka to prevent its binding to aPKC ► Crumbs outcompetes phospho-Baz for binding to PAR-6 in epithelial cells ► This mechanism defines the apicial/lateral boundary in epithelia ► Failure to exclude Baz from the aPKC complex disrupts epithelial morphogenesis
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影响因子：
4.6
作者：
Abdelilah-Seyfried, S;Cox, DN;Jan, YN
通讯作者：
Jan, YN
DOI：
10.1083/jcb.200807020
发表时间：
2008-12-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
St Johnston, Daniel
DOI：
10.1016/s0006-291x(02)02698-0
发表时间：
2002-12-13
影响因子：
3.1
作者：
Kohjima, M;Noda, Y;Sumimoto, H
通讯作者：
Sumimoto, H
影响因子：
6.6
作者：
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