Loss of reactivity to pan‐cadherin antibody in epidermal cells as a marker for metamorphic alteration of Xenopus skin
Loss of reactivity to pan‐cadherin antibody in epidermal cells as a marker for metamorphic alteration of Xenopus skin
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表皮细胞中泛钙粘蛋白抗体反应性丧失作为非洲爪蟾皮肤变质改变的标志
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Onoé
中科院分区：
文献类型：
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作者：
Y. Izutsu;S. Tochinai;K. Onoé
泛钙粘蛋白抗体识别细胞-细胞粘附分子钙粘蛋白家族的保守C末端区域，并对分子具有广谱反应性。在本研究中，通过使用抗泛钙粘蛋白单克隆抗体（mAb）的免疫组织化学，钙粘蛋白在表皮组织中的表达动态进行了分析，在变态的非洲爪蟾。在发育的早期阶段，抗pan cadherin mAb在细胞-细胞边界以及躯干和尾部表皮细胞的细胞质中检测到信号。在变态过程中，免疫反应下降，在躯干皮肤组织，但仍然在尾部。在顶极阶段，只在退化的尾部表皮中观察到免疫反应。信号从躯干表皮完全消失，已经转化为成人型组织。通过蛋白质印迹分析证实了这一观察结果。在幼虫皮肤中检测到特异性条带，但在成虫裂解物中未检测到，分子大小约为135 kDa，对应于钙粘蛋白的分子量。这种不同的免疫反应性在幼虫和成人中观察到的表皮的皮肤，但没有在任何其他组织检查，即，脑，肾和肝。在幼虫表皮细胞中观察到的免疫反应性在体外被甲状腺激素处理显著下调。这些免疫反应性变化对钙粘蛋白的C末端区域具有特异性，表明变态期间分子的细胞内改变，抗泛钙粘蛋白mAb可以作为适用于爪蟾变态分析的幼虫型表皮细胞的标记物。
Pan‐cadherin antibodies recognize the conserved C‐terminal region of the family of cell–cell adhesion molecules, cadherins, and have a broad spectrum of reactivity to the molecules. In the present study, by immunohistochemistry using an anti‐pan cadherin monoclonal antibody (mAb), expression dynamics of cadherins in epidermal tissues were analyzed during metamorphosis of Xenopus laevis. At early stages of development, the anti‐pan cadherin mAb detected signals at cell–cell boundaries and in the cytoplasm of both trunk and tail epidermal cells. During metamorphosis, the immunoreactivity decreased in the trunk skin tissue but remained in the tail. At the climax stage, immunoreactivity was observed only in the regressing tail epidermis. The signals disappeared completely from the trunk epidermis, which had already transformed into adult‐type tissue. This observation was confirmed by western blot analysis. A specific band was detected in the larval skin, but not in the adult lysate, at approximately 135 kDa in molecular size, corresponding to the molecular mass of cadherins. This different immunoreactivity in larvae and adults was observed in the epidermis of the skin, but not in any other tissues examined, that is, brain, kidney and liver. The immunoreactivity seen in larval epidermal cells was drastically downregulated by thyroid hormone treatment in vitro. These changes of immunoreactivity were specific for the C‐terminal region of cadherins, suggesting intracellular alteration of the molecules during metamorphosis, and the anti‐pan cadherin mAb can be a marker for larval‐type epidermal cells that is applicable to analysis of Xenopus metamorphosis.
非洲爪蟾发育过程中表皮角蛋白表达的空间、时间和激素调节。
影响因子：
2.7
作者：
Nishikawa,A;Shimizu-Nishikawa,K;Miller,L
通讯作者：
Miller,L
影响因子：
4.6
作者：
G. Levi;B. Gumbiner;J. Thiery
通讯作者：
G. Levi;B. Gumbiner;J. Thiery