Chromosome length and perinuclear attachment constrain resolution of DNA intertwines.
Chromosome length and perinuclear attachment constrain resolution of DNA intertwines.
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DOI：
10.1083/jcb.201404039
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发表时间：
2014-09-15
期刊：
影响因子：
--
通讯作者：
Mendoza M
中科院分区：
文献类型：
--
作者：
Titos I;Ivanova T;Mendoza M
Independent of the presence of rDNA repeats, topological constraints imposed by chromosome length and perinuclear attachment determine the efficiency with which sister chromatid intertwines are resolved by topoisomerase II and dynamic microtubules during anaphase. To allow chromosome segregation, topoisomerase II (topo II) must resolve sister chromatid intertwines (SCI) formed during deoxynucleic acid (DNA) replication. How this process extends to the full genome is not well understood. In budding yeast, the unique structure of the ribosomal DNA (rDNA) array is thought to cause late SCI resolution of this genomic region during anaphase. In this paper, we show that chromosome length, and not the presence of rDNA repeats, is the critical feature determining the time of topo II–dependent segregation. Segregation of chromosomes lacking rDNA also requires the function of topo II in anaphase, and increasing chromosome length aggravates missegregation in topo II mutant cells. Furthermore, anaphase Stu2-dependent microtubule dynamics are critical for separation of long chromosomes. Finally, defects caused by topo II or Stu2 impairment depend on attachment of telomeres to the nuclear envelope. We propose that topological constraints imposed by chromosome length and perinuclear attachment determine the amount of SCI that topo II and dynamic microtubules resolve during anaphase.
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影响因子：
16
作者：
Farcas AM;Uluocak P;Helmhart W;Nasmyth K
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