Cohesin's concatenation of sister DNAs maintains their intertwining.
Cohesin's concatenation of sister DNAs maintains their intertwining.
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DOI：
10.1016/j.molcel.2011.07.034
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发表时间：
2011-10-07
期刊：
影响因子：
16
通讯作者：
Nasmyth K
中科院分区：
文献类型：
--
作者：
Farcas AM;Uluocak P;Helmhart W;Nasmyth K
DNA链化对姐妹染色单体凝聚力的贡献尚不清楚，部分原因是它从未在有丝分裂染色体中直接观察到。差异沉降速度和凝胶电泳显示，姐妹的26 kb的圆形微型染色体举行在一起的连锁，以及由cohesin。粘着蛋白的三个亚基界面的化学交联捕获了环状而非线性微型染色体的姐妹DNA，这一发现意味着粘着蛋白使用拓扑原理发挥作用。重要的是，粘附素以这种方式将链连接和非链连接的DNA保持在一起。在着丝粒附近，索烃被纺锤体力分解，但即使在完全解链后，由粘着蛋白直接介导的连接也能抵抗这些力。重要的是，S期后连锁的持续性取决于粘着蛋白。我们的结论是，通过延缓拓扑II驱动的decatenation，cohesin介导姐妹染色单体凝聚力的间接机制，以及一个涉及其三环内的姐妹DNA的诱捕。在有丝分裂细胞中可以检测到姐妹染色单体的缠绕。通过臂位点以及着丝粒产生物理凝聚。姐妹染色单体的缠绕由粘着蛋白维持。粘着蛋白独立于SCI将姐妹染色单体保持在一起。
The contribution of DNA catenation to sister chromatid cohesion is unclear partly because it has never been observed directly within mitotic chromosomes. Differential sedimentation-velocity and gel electrophoresis reveal that sisters of 26 kb circular minichromosomes are held together by catenation as well as by cohesin. The finding that chemical crosslinking of cohesin's three subunit interfaces entraps sister DNAs of circular but not linear minichromosomes implies that cohesin functions using a topological principle. Importantly, cohesin holds both catenated and uncatenated DNAs together in this manner. In the vicinity of centromeres, catenanes are resolved by spindle forces, but linkages mediated directly by cohesin resist these forces even after complete decatenation. Crucially, persistence of catenation after S phase depends on cohesin. We conclude that by retarding Topo II-driven decatenation, cohesin mediates sister chromatid cohesion by an indirect mechanism as well as one involving entrapment of sister DNAs inside its tripartite ring. ► Sister chromatid intertwining can be detected in mitotic cells ► Physical cohesion is generated through arm loci as well as centromeres ► Sister chromatid intertwining is maintained by cohesin ► Cohesin holds sister chromatids together independent of SCI
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影响因子：
16
作者：
Haering, CH;Löwe, J;Nasmyth, K
通讯作者：
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