Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou
Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou
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中国小麦地方品种葫芦头白粉病抗性基因MlHLT的遗传与物理定位
DOI：
10.1007/s00122-014-2436-2
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Wang Zhenzhong, Hanwen Li, Deyun Zhang, Li Guo, Jiaojiao Chen
中科院分区：
文献类型：
--
作者：
Wang Zhenzhong, Hanwen Li, Deyun Zhang, Li Guo, Jiaojiao Chen
来源于中国小麦地方品种的抗白粉病基因MlHLT在染色体1DS上的一个3.6厘摩（cM）遗传区间内跨越13.4兆碱基（Mb）的物理基因组区域。小麦白粉病是由小麦白粉病菌Blumeria graminis f.小麦赤星病菌（Bgt）是一种毁灭性的病害，可导致严重的产量损失。中国小麦地方品种葫芦头对北京地区流行的Bgt分离物E09具有近免疫抗性。遗传分析表明葫芦头的抗白粉病性受一对显性基因控制，暂定名为MlHLT。群体分离分析（BSA）和简单重复序列（SSR）分析表明，MlHLT位于染色体臂1DS上，位于标记Xgwm 337和Xcfd 83/Xcfd 72之间。通过比较基因组学分析，将节节麦1DS染色体上的MlHLT位点的共线性基因组区域分别定位在二穗短柄草2号染色体、水稻5号染色体和高粱9号染色体上。利用Ae的基因组草图序列和扩展的单核苷酸多态性（SNP）标记序列，开发了3个新的多态性标记。tauschii登录号AL 8/78，以及普通小麦cv.中国春454重叠群序列和国际小麦基因组测序联盟（IWGSC）调查序列。MlHLT定位于跨越13.4Mb物理基因组区域的3.6cM遗传区间，包含7个重叠群（ctg 220、ctg 4623、ctg 1063、ctg 5929、ctg 3163、ctg 699和ctg 1065）与短柄草属植物369.8kb基因组区域具有同线性，水稻中的380.8kb基因组区域和高粱中的298.4kb基因组区域。MlHLT的遗传图谱和物理图谱为葫芦头白粉病抗性基因MlHLT的图位克隆和分子标记辅助选择（MAS）提供了框架。
A powdery mildew resistance gene MlHLT derived from a Chinese wheat landrace maps within a 3.6 centimorgan (cM) genetic interval spanning a 13.4 megabase (Mb) physical genomic region on chromosome 1DS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt) is a devastating disease that can cause severe yield losses. Chinese wheat landrace Hulutou confers nearly immune resistance against prevailing Bgt isolate E09 in Beijing. Genetic analysis indicate that the powdery mildew resistance of Hulutou is controlled by a single dominant gene, provisionally designated MlHLT. Bulked segregant analysis(BSA) and simple sequence repeat (SSR) mapping showed that MlHLT is located on chromosome arm 1DS between markers Xgwm337 and Xcfd83/Xcfd72. By applying comparative genomics analysis, collinearity genomic regions of the MlHLT locus on Aegilops tauschii chromosome 1DS were identified in Brachypodium distachyon chromosome 2, rice chromosome 5 and sorghum chromosome 9, respectively. Three new polymorphic markers were developed using the draft genome sequences and the extended single nucleotide polymorphism (SNP) marker sequences of Ae. tauschii accession AL8/78, as well as the Triticum aestivum cv. Chinese Spring 454 contig sequences and the International Wheat Genome Sequencing Consortium (IWGSC) survey sequences. MlHLT mapped into a 3.6 cM genetic interval spanning 13.4 Mb physical genomic region containing seven contigs (ctg220, ctg4623, ctg1063, ctg5929, ctg3163, ctg699 and ctg1065) on 1DS that has synteny with a 369.8 kb genomic region in Brachypodium, a 380.8 kb genomic region in rice and a 298.4 kb genomic region in sorghum. The genetic and physical maps of MlHLT provide framework for map-based cloning and marker-assisted selection (MAS) of the powdery mildew resistance gene MlHLT in Hulutou.
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