The nucleus acts as a ruler tailoring cell responses to spatial constraints.
The nucleus acts as a ruler tailoring cell responses to spatial constraints.
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DOI：
10.1126/science.aba2894
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发表时间：
2020-10-16
期刊：
影响因子：
--
通讯作者：
Piel M
中科院分区：
文献类型：
--
作者：
Lomakin AJ;Cattin CJ;Cuvelier D;Alraies Z;Molina M;Nader GPF;Srivastava N;Sáez PJ;Garcia-Arcos JM;Zhitnyak IY;Bhargava A;Driscoll MK;Welf ES;Fiolka R;Petrie RJ;De Silva NS;González-Granado JM;Manel N;Lennon-Duménil AM;Müller DJ;Piel M
后生动物体内的微观环境非常拥挤。单个细胞是否能够根据有限的空间调整其行为仍不清楚。在这里，我们发现细胞使用其最大、最坚硬的细胞器——细胞核来测量空间限制的程度。低于静息细胞核尺寸的细胞限制会使细胞核变形，从而扩张并拉伸其包膜。这会通过核膜拉伸敏感蛋白激活向肌动球蛋白皮质发出的信号，从而上调细胞收缩性。我们确定，定制的收缩反应构成了涉及迁移细胞行为的基于核标尺的信号通路。细胞依靠核统治者来调节动力，使其能够通过复杂三维 (3D) 环境中的限制性孔道，这一过程与癌细胞入侵、免疫反应和胚胎发育相关。核膜扩张超过阈值会触发收缩细胞反应，从而充当细胞变形程度的标尺。
The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. Here, we found that cells measure the degree of spatial confinement using their largest and stiffest organelle, the nucleus. Cell confinement below a resting nucleus size deforms the nucleus, which expands and stretches its envelope. This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, upregulating cell contractility. We established that the tailored contractile response constitutes a nuclear ruler-based signaling pathway involved in migratory cell behaviors. Cells rely on the nuclear ruler to modulate the motive force enabling their passage through restrictive pores in complex three-dimensional (3D) environments, a process relevant to cancer cell invasion, immune responses and embryonic development. Nuclear envelope expansion above a threshold triggers a contractile cell response and thus acts as a ruler for the degree of cell deformation.
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DOI：
10.1073/pnas.1702226114
发表时间：
2017-09-26
影响因子：
11.1
作者：
Chu, Fang-Yi;Haley, Shannon C.;Zidovska, Alexandra
通讯作者：
Zidovska, Alexandra
影响因子：
4
作者：
Ma, Yan;Cai, Shang;Zhang, Chuanmao
通讯作者：
Zhang, Chuanmao
影响因子：
2.5
作者：
Le Berre, Mael;Aubertin, Johannes;Piel, Matthieu
通讯作者：
Piel, Matthieu
DOI：
10.1083/jcb.200810002
发表时间：
2009-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kong F;García AJ;Mould AP;Humphries MJ;Zhu C
通讯作者：
Zhu C
影响因子：
3.4
作者：
Dean, Kevin M.;Roudot, Philippe;Welf, Erik S.;Danuser, Gaudenz;Fiolka, Reto
通讯作者：
Fiolka, Reto