Comparing the MRI appearance of the lymph nodes and spleen in wild-type and immuno-deficient mouse strains.
Comparing the MRI appearance of the lymph nodes and spleen in wild-type and immuno-deficient mouse strains.
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DOI：
10.1371/journal.pone.0027508
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Foster PJ
中科院分区：
文献类型：
--
作者：
Economopoulos V;Noad JC;Krishnamoorthy S;Rutt BK;Foster PJ
本研究的目的是调查研究中常用的免疫功能正常和免疫缺陷小鼠的淋巴器官的正常 MRI 外观。四种不同小鼠品系（裸鼠、NOG、C57BL/6、CB-17 SCID (SCID)）各四只小鼠每周进行一次成像，持续一个月。使用 3D 平衡稳态自由进动 (bSSFP) 序列获取图像。通过 MR 图像测量淋巴结和脾脏的体积。在裸鼠和 SCID 小鼠的图像中，淋巴结有时包含 MRI 图像上可见的高信号区域。裸鼠中节点的体积变化很大。 SCID 小鼠的淋巴结比裸鼠或 C57Bl/6 小鼠的淋巴结小 (p<0.0001)。所有菌株的淋巴结体积随时间略有变化。 C57Bl/6和裸鼠的脾脏大小和外观相似。 SCID 和 NOG 小鼠的脾脏明显较小（p<0.0001）并且外观异常。本研究中检查的不同小鼠品系的正常淋巴结和脾脏的 MRI 外观差异很大。当这些菌株用于 MRI 成像研究时，认识到这一点很重要，以避免将 MRI 结果误解为异常。
The goal of this study was to investigate the normal MRI appearance of lymphoid organs in immuno-competent and immuno-deficient mice commonly used in research. Four mice from each of four different mouse strains (nude, NOG, C57BL/6, CB-17 SCID (SCID)) were imaged weekly for one month. Images were acquired with a 3D balanced steady state free precession (bSSFP) sequence. The volume of the lymph nodes and spleens were measured from MR images. In images of nude and SCID mice, lymph nodes sometimes contained a hyperintense region visible on MRI images. Volumes of the nodes were highly variable in nude mice. Nodes in SCID mice were smaller than in nude or C57Bl/6 mice (p<0.0001). Lymph node volumes changed slightly over time in all strains. The spleens of C57Bl/6 and nude mice were similar in size and appearance. Spleens of SCID and NOG mice were significantly smaller (p<0.0001) and abnormal in appearance. The MRI appearance of the normal lymph nodes and spleen varies considerably in the various mouse strains examined in this study. This is important to recognize in order to avoid the misinterpretation of MRI findings as abnormal when these strains are used in MRI imaging studies.
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影响因子：
11.2
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
SPANGTHOMSEN, M