Identification of a novel transport system in Borrelia burgdorferi that links the inner and outer membranes.
Identification of a novel transport system in Borrelia burgdorferi that links the inner and outer membranes.
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DOI：
10.1093/femspd/ftad014
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发表时间：
2023-01-17
影响因子：
3.3
通讯作者：
Akins, Darrin R.
中科院分区：
文献类型：
--
作者：
Bowen, Hannah G.;Kenedy, Melisha R.;Johnson, David K.;MacKerell Jr, Alexander D.;Akins, Darrin R.
关键词：
伯氏疏螺旋体是导致莱姆病的螺旋体，它是一种双层生物体，与革兰氏阴性生物体相似，因为它含有内膜和外膜。然而，与典型的革兰氏阴性菌不同，B. burgdorferi缺乏脂多糖（LPS）。使用计算基因组分析和结构建模，我们确定了一个运输系统，包含六个蛋白质在B。burgdorferi，它们都是在脂多糖转运（LPT）系统中发现的蛋白质的直系同源物，所述脂多糖转运（LPT）系统连接革兰氏阴性生物体的内膜和外膜并且负责将LPS置于这些生物体的表面上。而B.伯氏疏螺旋体不含LPS，但它编码超过100种不同的表面暴露脂蛋白和几种主要糖脂，它们与LPS一样也是高度两亲性分子，尽管还不知道将这些分子转运到疏螺旋体表面的系统。因此，进行了补充分子建模的实验以确定在B中是否鉴定了正向LPT系统。伯氏疏螺旋体可将脂蛋白和/或糖脂转运至疏螺旋体外膜。我们的综合观察强烈表明，LPT转运系统不运输脂蛋白的表面。然而，分子动力学建模表明疏螺旋体LPT系统可以将疏螺旋体糖脂转运到外膜。在莱姆病螺旋体中发现了一种新的转运途径。
Borrelia burgdorferi, the spirochete that causes Lyme disease, is a diderm organism that is similar to Gram-negative organisms in that it contains both an inner and outer membrane. Unlike typical Gram-negative organisms, however, B. burgdorferi lacks lipopolysaccharide (LPS). Using computational genome analyses and structural modeling, we identified a transport system containing six proteins in B. burgdorferi that are all orthologs to proteins found in the lipopolysaccharide transport (LPT) system that links the inner and outer membranes of Gram-negative organisms and is responsible for placing LPS on the surface of these organisms. While B. burgdorferi does not contain LPS, it does encode over 100 different surface-exposed lipoproteins and several major glycolipids, which like LPS are also highly amphiphilic molecules, though no system to transport these molecules to the borrelial surface is known. Accordingly, experiments supplemented by molecular modeling were undertaken to determine whether the orthologous LPT system identified in B. burgdorferi could transport lipoproteins and/or glycolipids to the borrelial outer membrane. Our combined observations strongly suggest that the LPT transport system does not transport lipoproteins to the surface. Molecular dynamic modeling, however, suggests that the borrelial LPT system could transport borrelial glycolipids to the outer membrane. A novel transport pathway has been identified in the Lyme disease spirochete.
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影响因子：
16.6
作者：
Botte M;Ni D;Schenck S;Zimmermann I;Chami M;Bocquet N;Egloff P;Bucher D;Trabuco M;Cheng RKY;Brunner JD;Seeger MA;Stahlberg H;Hennig M
通讯作者：
Hennig M
DOI：
10.1126/science.1227215
发表时间：
2012-09-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chng SS;Xue M;Garner RA;Kadokura H;Boyd D;Beckwith J;Kahne D
通讯作者：
Kahne D
DOI：
10.4269/ajtmh.2011.11-0292
发表时间：
2011-12-01
影响因子：
3.3
作者：
Dolan, Marc C.;Schulze, Terry L.;Piesman, Joseph
通讯作者：
Piesman, Joseph
影响因子：
3.1
作者：
Brooks, CS;Vuppala, SR;Akins, DR
通讯作者：
Akins, DR
影响因子：
4.4
作者：
Brooks, CS;Vuppala, SR;Akins, DR
通讯作者：
Akins, DR