A Review on Host-Leptospira Interactions: What We Know and Future Expectations.
A Review on Host-Leptospira Interactions: What We Know and Future Expectations.
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DOI：
10.3389/fcimb.2021.777709
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发表时间：
2021
影响因子：
5.7
通讯作者：
Nascimento ALTO
中科院分区：
文献类型：
--
作者：
Daroz BB;Fernandes LGV;Cavenague MF;Kochi LT;Passalia FJ;Takahashi MB;Nascimento Filho EG;Teixeira AF;Nascimento ALTO
钩端螺旋体病是由致病性钩端螺旋体引起的一种广泛存在的人畜共患病，被认为是人类和兽医关注的一种被忽视的传染病。我们的小组一直在研究被标注为假设的蛋白质，预测位于钩端螺旋体表面。由于它们的位置，这些蛋白质可能具有与各种宿主成分相互作用的能力，这可能导致感染的建立。这些蛋白通过结合宿主受体分子（如细胞外基质（ECM）成分层粘连蛋白和糖胺聚糖）作为粘附因子，帮助细菌定植。钩端螺旋体还与宿主纤维蛋白溶解系统相互作用，这已被证明是入侵机制的有力工具。与纤维蛋白原和凝血酶的相互作用已被证明可减少纤维蛋白凝块的形成。此外，分泌的蛋白酶或获得性表面纤溶酶对凝血级联成分的降解也可能在减少血块形成中发挥作用，从而促进感染期间的传播。与宿主补体系统调节因子的相互作用也在帮助细菌逃避免疫系统，促进入侵中发挥作用。钩端螺旋体与细胞受体（如钙粘蛋白）的相互作用有助于研究参与毒力的分子。为了更好地了解宿主-病原体相互作用，钩端螺旋体诱变工具已经开发和探索。本文介绍了几种介导与ECM、血浆、补体系统和细胞结合的蛋白质，以收集有助于更好地理解钩端螺旋体与宿主相互作用机制的研究成果，并讨论了旨在验证蛋白质功能的钩端螺旋体的遗传操作。
Leptospirosis is a widespread zoonosis caused by pathogenic Leptospira spp. It is considered a neglected infectious disease of human and veterinary concern. Our group has been investigating proteins annotated as hypothetical, predicted to be located on the leptospiral surface. Because of their location, these proteins may have the ability to interact with various host components, which could allow establishment of the infection. These proteins act as adherence factors by binding to host receptor molecules, such as the extracellular matrix (ECM) components laminin and glycosaminoglycans to help bacterial colonization. Leptospira also interacts with the host fibrinolytic system, which has been demonstrated to be a powerful tool for invasion mechanisms. The interaction with fibrinogen and thrombin has been shown to reduce fibrin clot formation. Additionally, the degradation of coagulation cascade components by secreted proteases or by acquired surface plasmin could also play a role in reducing clot formation, hence facilitating dissemination during infection. Interaction with host complement system regulators also plays a role in helping bacteria to evade the immune system, facilitating invasion. Interaction of Leptospira to cell receptors, such as cadherins, can contribute to investigate molecules that participate in virulence. To achieve a better understanding of the host-pathogen interaction, leptospiral mutagenesis tools have been developed and explored. This work presents several proteins that mediate binding to components of the ECM, plasma, components of the complement system and cells, to gather research achievements that can be helpful in better understanding the mechanisms of leptospiral-host interactions and discuss genetic manipulation for Leptospira spp. aimed at protein function validation.
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影响因子：
3.8
作者：
Breda LC;Hsieh CL;Castiblanco Valencia MM;da Silva LB;Barbosa AS;Blom AM;Chang YF;Isaac L
通讯作者：
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通讯作者：
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影响因子：
2.1
作者：
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通讯作者：
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