Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant-pathogen interactions in a tree.
Association mapping, transcriptomics, and transient expression identify candidate genes mediating plant-pathogen interactions in a tree.
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DOI：
10.1073/pnas.1804428115
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发表时间：
2018-11-06
影响因子：
11.1
通讯作者：
LeBoldus JM
中科院分区：
文献类型：
--
作者：
Muchero W;Sondreli KL;Chen JG;Urbanowicz BR;Zhang J;Singan V;Yang Y;Brueggeman RS;Franco-Coronado J;Abraham N;Yang JY;Moremen KW;Weisberg AJ;Chang JH;Lindquist E;Barry K;Ranjan P;Jawdy S;Schmutz J;Tuskan GA;LeBoldus JM
国际贸易导致植物病害进入世界各地的自然生态系统。这些引入对生态系统结构和功能具有潜在的灾难性影响。利用基因组工具、树种内的自然变异和高通量表型平台，我们提出了一个可广泛应用于快速识别与引入植物病害的抗性和易感性相关的候选基因的框架。在木本树木中鉴定候选基因的速度和准确性前所未有，这证明了基因组学在减轻入侵性疾病对森林健康影响方面的潜力。引起橡树突然死亡等疾病的入侵微生物对世界各地的生态系统和经济产生负面影响。用于对抗外来疾病的抗性基因型的部署通常依赖于可能需要数十年才能完成的育种计划。为了演示如何加速这一过程，我们采用了约的全基因组关联图谱。 1,000 棵经过重新测序的毛果杨树分别受到一种入侵性真菌病原体 Sphaerulina musiva 的攻击。在显着关联中，鉴定出三个与耐药性相关的位点，并预测它们编码一种假定的膜结合 L 型受体样激酶和两种受体样蛋白。预计与易感性相关的位点编码假定的 G 型 D-甘露糖结合受体样激酶。包括等位基因分析、转录组学、结合测定和过表达在内的多种证据支持这些候选基因在毛果松对 S. musiva 反应中的假设功能。
International trade has resulted in the introduction of plant diseases into natural ecosystems around the world. These introductions have potentially catastrophic impacts on ecosystem structure and function. Leveraging genomic tools, natural variation within a tree species, and a high-throughput phenotyping platform, we present a framework that can be broadly applied to rapidly identify candidate genes associated with resistance and susceptibility to introduced plant diseases. The unprecedented speed and accuracy with which the candidate genes can be identified in woody trees demonstrates the potential of genomics to mitigate the impacts of invasive diseases on forest health. Invasive microbes causing diseases such as sudden oak death negatively affect ecosystems and economies around the world. The deployment of resistant genotypes for combating introduced diseases typically relies on breeding programs that can take decades to complete. To demonstrate how this process can be accelerated, we employed a genome-wide association mapping of ca. 1,000 resequenced Populus trichocarpa trees individually challenged with Sphaerulina musiva, an invasive fungal pathogen. Among significant associations, three loci associated with resistance were identified and predicted to encode one putative membrane-bound L-type receptor-like kinase and two receptor-like proteins. A susceptibility-associated locus was predicted to encode a putative G-type D-mannose–binding receptor-like kinase. Multiple lines of evidence, including allele analysis, transcriptomics, binding assays, and overexpression, support the hypothesized function of these candidate genes in the P. trichocarpa response to S. musiva.
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影响因子：
5.6
作者：
Lannoo N;Van Damme EJ
通讯作者：
Van Damme EJ
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
15
作者：
Chundawat, Shishir P. S.;Bellesia, Giovanni;Dale, Bruce E.
通讯作者：
Dale, Bruce E.
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
果皮同质分离剂掩盖了植物细胞壁中丰富的木葡聚糖表位。
影响因子：
5.3
作者：
Marcus SE;Verhertbruggen Y;Hervé C;Ordaz-Ortiz JJ;Farkas V;Pedersen HL;Willats WG;Knox JP
通讯作者：
Knox JP