Best practices for managing large CryoEM facilities.

Best practices for managing large CryoEM facilities.
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DOI:
10.1016/j.jsb.2017.07.011
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发表时间:
2017-09
影响因子:
3
通讯作者:
Potter CS
Potter CS
中科院分区:
生物学3区
文献类型:
--
作者:
Alewijnse B;Ashton AW;Chambers MG;Chen S;Cheng A;Ebrahim M;Eng ET;Hagen WJH;Koster AJ;López CS;Lukoyanova N;Ortega J;Renault L;Reyntjens S;Rice WJ;Scapin G;Schrijver R;Siebert A;Stagg SM;Grum-Tokars V;Wright ER;Wu S;Yu Z;Zhou ZH;Carragher B;Potter CS

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本文概述了2017年2月6-7日在纽约纽约结构生物学中心举行的大型低温EM设施管理研讨会的讨论和演讲。讲习班的一个主要目标是讨论管理低温EM设施的最佳做法。讨论主要集中在支持用于低温EM的单粒子方法,主题包括:用户准入、评估项目、工作流程、样品处理、显微镜、数据管理和处理以及用户培训。
This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.
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