The genomic basis of circadian and circalunar timing adaptations in a midge.
The genomic basis of circadian and circalunar timing adaptations in a midge.
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DOI：
10.1038/nature20151
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发表时间：
2016-12-01
期刊：
影响因子：
64.8
通讯作者：
Tessmar-Raible, Kristin
中科院分区：
文献类型：
--
作者：
Kaiser, Tobias S.;Poehn, Birgit;Szkiba, David;Preussner, Marco;Sedlazeck, Fritz J.;Zrim, Alexander;Neumann, Tobias;Lam-Tung Nguyen;Betancourt, Andrea J.;Hummel, Thomas;Vogel, Heiko;Dorner, Silke;Heyd, Florian;von Haeseler, Arndt;Tessmar-Raible, Kristin
生物体利用内源时钟来预测规律的环境周期，例如白天和潮汐。导致不同时间行为或生理学的自然变异（称为人类的时间型）尚未在分子水平上得到很好的表征。我们对 Clunio marinus 的基因组进行了测序，这是一种海洋蠓，其繁殖由昼夜节律和周月钟计时。来自不同地点的蠓表现出品系特异性的遗传时间适应。我们检查了来自不同地点的五种 C. marinus 菌株的遗传变异，并绘制了圆周和昼夜节律时钟型的数量性状基因座。与昼夜节律时间型最密切相关的区域在钙/钙调蛋白依赖性激酶 II.1 (CaMKII.1) 剪接变体的丰度方面产生菌株特异性差异。由于同等变体被证明可以改变果蝇中的 CaMKII 活性，而 C. marinus (Cma)-CaMKII.1 会增加昼夜节律蛋白 Cma-CLOCK 和 Cma-CYCLE 二聚体的转录活性，因此我们认为选择性剪接的调节是昼夜节律时间自然适应的机制。本文的在线版本 (doi:10.1038/nature20151) 包含补充材料，可供授权用户使用。 Clunio marinus 计时菌株的基因组和分子分析表明，Ca2+/钙调蛋白依赖性激酶 II 选择性剪接的调节代表了昼夜节律计时进化适应的机制。本文的在线版本 (doi:10.1038/nature20151) 包含补充材料，可供授权用户使用。 Kristin Tessmar-Raible 及其同事报告了 Clunio marinus 的基因组，这是一种海洋蠓，其繁殖根据昼夜节律和周月钟与潮汐同步。为了确定与时间差异相关的遗传变异，作者报告了一系列具有一系列昼夜节律和周月时间的 C. marinus 菌株的遗传图谱。他们认为，昼夜节律和昼夜节律是由不同的途径调节的，没有发现核心时钟基因的参与，并且暗示钙/钙调蛋白依赖性激酶 II.1 参与了昼夜节律的调节。本文的在线版本 (doi:10.1038/nature20151) 包含补充材料，可供授权用户使用。
Organisms use endogenous clocks to anticipate regular environmental cycles, such as days and tides. Natural variants resulting in differently timed behaviour or physiology, known as chronotypes in humans, have not been well characterized at the molecular level. We sequenced the genome of Clunio marinus, a marine midge whose reproduction is timed by circadian and circalunar clocks. Midges from different locations show strain-specific genetic timing adaptations. We examined genetic variation in five C. marinus strains from different locations and mapped quantitative trait loci for circalunar and circadian chronotypes. The region most strongly associated with circadian chronotypes generates strain-specific differences in the abundance of calcium/calmodulin-dependent kinase II.1 (CaMKII.1) splice variants. As equivalent variants were shown to alter CaMKII activity in Drosophila melanogaster, and C. marinus (Cma)-CaMKII.1 increases the transcriptional activity of the dimer of the circadian proteins Cma-CLOCK and Cma-CYCLE, we suggest that modulation of alternative splicing is a mechanism for natural adaptation in circadian timing. The online version of this article (doi:10.1038/nature20151) contains supplementary material, which is available to authorized users. Genomic and molecular analyses of Clunio marinus timing strains suggest that modulation of alternative splicing of Ca2+/calmodulin-dependent kinase II represents a mechanism for evolutionary adaptation of circadian timing. The online version of this article (doi:10.1038/nature20151) contains supplementary material, which is available to authorized users. Kristin Tessmar-Raible and colleagues report the genome of Clunio marinus, a marine midge whose reproduction is timed to the tides by circadian and circalunar clocks. To identify genetic variation associated with timing differences, the authors report genetic mapping in a selection of C. marinus strains with a range of circadian and circalunar timing. They suggest that circalunar and circadian timing are regulated by separate pathways, do not find involvement of core clock genes, and implicate calcium/calmodulin-dependent kinase II.1 in the regulation of circadian timing. The online version of this article (doi:10.1038/nature20151) contains supplementary material, which is available to authorized users.
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影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.0707772105
发表时间：
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影响因子：
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作者：
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