Designed and biologically active protein lattices.
Designed and biologically active protein lattices.
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DOI：
10.1038/s41467-021-23966-4
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发表时间：
2021-06-17
影响因子：
16.6
通讯作者：
Gang O
中科院分区：
文献类型：
--
作者：
Wang ST;Minevich B;Liu J;Zhang H;Nykypanchuk D;Byrnes J;Liu W;Bershadsky L;Liu Q;Wang T;Ren G;Gang O
在空间中组织蛋白质的通用方法需要使复杂的生物材料，工程生物分子支架，无细胞生物学和混合纳米级系统成为可能。在这里，我们展示了如何定制的封装蛋白质在DNA为基础的体素可以与可编程的组装，指导这些体素到生物功能蛋白质阵列与规定的和有序的二维（2D）和三维（3D）组织相结合。我们将所提出的概念应用于铁蛋白（一种铁储存蛋白）及其无铁类似物脱铁铁蛋白（apoferritin），以形成单层、双层以及几种类型的3D蛋白质晶格。我们的研究表明，内部体素设计和体素间编码可以有效地用于创建具有设计组织的蛋白质晶格，正如原位X射线散射和冷冻电子显微镜3D成像所证实的那样。组装的蛋白质阵列在与蛋白质功能相关的环境中保持结构稳定性和生物活性。然后，阵列的框架设计允许小分子进入铁蛋白及其铁核心，并通过释放铁离子将其转化为脱铁铁蛋白阵列。该研究介绍了一种平台方法，用于创建具有所需2D和3D组织的生物活性含蛋白质的有序纳米材料。在2D和3D中组织蛋白质是开发用于一系列应用的复杂双分子材料所必需的。在这里，作者报告了铁蛋白和脱铁铁蛋白在基于DNA的体素中的封装，并通过编程组装生成2D和3D蛋白质晶格，并证明蛋白质功能的保留。
Versatile methods to organize proteins in space are required to enable complex biomaterials, engineered biomolecular scaffolds, cell-free biology, and hybrid nanoscale systems. Here, we demonstrate how the tailored encapsulation of proteins in DNA-based voxels can be combined with programmable assembly that directs these voxels into biologically functional protein arrays with prescribed and ordered two-dimensional (2D) and three-dimensional (3D) organizations. We apply the presented concept to ferritin, an iron storage protein, and its iron-free analog, apoferritin, in order to form single-layers, double-layers, as well as several types of 3D protein lattices. Our study demonstrates that internal voxel design and inter-voxel encoding can be effectively employed to create protein lattices with designed organization, as confirmed by in situ X-ray scattering and cryo-electron microscopy 3D imaging. The assembled protein arrays maintain structural stability and biological activity in environments relevant for protein functionality. The framework design of the arrays then allows small molecules to access the ferritins and their iron cores and convert them into apoferritin arrays through the release of iron ions. The presented study introduces a platform approach for creating bio-active protein-containing ordered nanomaterials with desired 2D and 3D organizations. Organising proteins in 2D and 3D is needed to develop complex bimolecular materials for a range of applications. Here, the authors report the encapsulation of ferritin and apoferritin in DNA-based voxels with programmed assembly to generate both 2D and 3D protein lattices and demonstrate the retention of protein function.
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DOI：
10.1073/pnas.1503533112
发表时间：
2015-04-14
影响因子：
11.1
作者：
Brodin, Jeffrey D.;Auyeung, Evelyn;Mirkin, Chad A.
通讯作者：
Mirkin, Chad A.
DOI：
10.1107/s0907444997003314
发表时间：
1997-09-01
影响因子：
2.2
作者：
Granier, T;Gallois, B;Precigoux, G
通讯作者：
Precigoux, G
DOI：
10.1002/wnan.1657
发表时间：
2021-01
期刊：
Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
影响因子：
--
作者：
Dobrovolskaia MA;Bathe M
通讯作者：
Bathe M
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
64.8
作者：
Benson, Erik;Mohammed, Abdulmelik;Hogberg, Bjorn
通讯作者：
Hogberg, Bjorn