Co-localization of corticotropin releasing factor and vasopressin mRNA in neurones after adrenalectomy
Co-localization of corticotropin releasing factor and vasopressin mRNA in neurones after adrenalectomy
复制标题
肾上腺切除术后促肾上腺皮质激素释放因子和加压素 mRNA 在神经元中的共定位
作者：
B. Wolfson;R. W. Manning;L. G. Davis;R. Arentzen;F. Baldino
大鼠肾上腺切除后，精氨酸加压素和促肾上腺皮质激素释放因子(CRF)免疫反应性在下丘脑室旁核(PVN)的离散解剖分布发生改变。不仅这两种多肽的免疫染色增强，而且加压素免疫反应性(通常仅限于大细胞亚区)在很大比例的CRF神经元中变得清晰。这些免疫反应性的变化可能反映了翻译后事件、肽代谢或基因组活性的变化，这些变化间接或直接导致了加压素表达的增强。在这里，我们报告了肾上腺切除后，PVN中与加压素信使RNA同源的转录本水平增加，同时加压素免疫反应性增加。事实上，肾上腺切除后，可以在CRF免疫反应神经元中检测到加压素mRNA。这些结果表明，成年大鼠神经元基因组保留了相当程度的可塑性，这种可塑性可能受到内分泌环境的调节。
The discrete anatomical distribution of arginine vasopressin and corticotropin releasing factor (CRF) immunoreactivity in the para-ventricular nucleus (PVN) of the rat hypothalamus is altered after adrenalectomy1–3. Not only is the immunostaining of both peptides enhanced, but vasopressin immunoreactivity, normally confined to the magnocellular subdivision, becomes clear in a large percentage of CRF neurones in the parvocellular subdivision2. These changes in immunoreactivity may reflect changes in post-translational events, peptide metabolism or genomic activity that lead indirectly or directly to the enhanced expression of vasopressin. Here we report that levels of transcripts homologous to vasopressin messenger RNA increase in the PVN after adrenalectomy, in parallel with increases in vasopressin immunoreactivity. In fact, after adrenalectomy, vasopressin mRNA can be detected in CRF-immunoreactive neurones. These results indicate that a considerable degree of plasticity is retained by the adult neuronal genome of the rat and that this plasticity may be modulated by the endocrine environment.
影响因子：
4.1
作者：
SWANSON, LW;SAWCHENKO, PE;VALE, WW
通讯作者：
VALE, WW
影响因子：
4.8
作者：
RIVIER, C;VALE, W
通讯作者：
VALE, W