Estrogen receptor α and G-protein coupled estrogen receptor 1 are localized to GABAergic neurons in the dorsal striatum.
Estrogen receptor α and G-protein coupled estrogen receptor 1 are localized to GABAergic neurons in the dorsal striatum.
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DOI：
10.1016/j.neulet.2016.04.023
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发表时间：
2016-05-27
影响因子：
2.5
通讯作者：
Brake WG
中科院分区：
文献类型：
--
作者：
Almey A;Milner TA;Brake WG
雌激素影响纹状体中的多巴胺传递，增加多巴胺可用性，维持D2受体密度，并降低多巴胺转运蛋白的可用性。雌激素的这些作用中的一些是快速的，这表明它们是由膜相关受体介导的。最近我们的研究小组发现，在纹状体中存在ERα、ERβ和GPER 1的核外标记，但ERα和GPER 1不定位于该区域的多巴胺能神经元。GABA能神经元是纹状体中最常见的神经元类型，GABA传递的变化影响多巴胺传递。因此，为了确定ERα或GPER 1是否定位于GABA能神经元，我们用ERα或GPER 1和GABA的抗体双重标记纹状体，并使用电子显微镜检查它们。超微结构分析显示，ERα和GPER 1仅定位于纹状体的胞核部位，约35%的树突和轴突终末含有GABA免疫反应性。结合在膜相关的ERα和GPER 1可以解释快速雌激素诱导的纹状体中GABA传递的减少，这反过来又可以影响该区域的多巴胺传递。
Estrogens affect dopamine transmission in the striatum, increasing dopamine availability, maintaining D2 receptor density, and reducing the availability of the dopamine transporter. Some of these effects of estrogens are rapid, suggesting that they are mediated by membrane associated receptors. Recently our group demonstrated that there is extra-nuclear labeling for ERα, ERβ, and GPER1 in the striatum, but that ERα and GPER1 are not localized to dopaminergic neurons in this region. GABAergic neurons are the most common type of neuron in the striatum, and changes in GABA transmission affect dopamine transmission. Thus, to determine whether ERα or GPER1 are localized to GABAergic neurons, we double labeled the striatum with antibodies for ERα or GPER1 and GABA and examined them using electron microscopy. Ultrastructural analysis revealed that ERα and GPER1 are localized exclusively to extranuclear sites in the striatum, and ~35% of the dendrites and axon terminals labeled for these receptors contain GABA immunoreactivity. Binding at membrane-associated ERα and GPER1 could account for rapid estrogen-induced decreases in GABA transmission in the striatum, which, in turn, could affect dopamine transmission in this region.
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影响因子：
2.9
作者：
Hammond R;Gibbs RB
通讯作者：
Gibbs RB
影响因子：
2.3
作者：
Hu, M;Watson, CJ;Becker, JB
通讯作者：
Becker, JB
影响因子：
4.1
作者：
Landry, M;Lévesque, D;Di Paolo, T
通讯作者：
Di Paolo, T
DOI：
10.1523/jneurosci.4647-08.2009
发表时间：
2009-02-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Schultz KN;von Esenwein SA;Hu M;Bennett AL;Kennedy RT;Musatov S;Toran-Allerand CD;Kaplitt MG;Young LJ;Becker JB
通讯作者：
Becker JB
影响因子：
3.4
作者：
Gundersen, V;Ottersen, OP;StormMathisen, J
通讯作者：
StormMathisen, J