An NMR-based metabolomic approach to investigate the effects of supplementation with glutamic acid in piglets challenged with deoxynivalenol.
An NMR-based metabolomic approach to investigate the effects of supplementation with glutamic acid in piglets challenged with deoxynivalenol.
复制标题
一种基于NMR的代谢组方法，用于研究用脱氧烯醇挑战的小猪中补充谷氨酸的影响。
DOI：
10.1371/journal.pone.0113687
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Wu M;Xiao H;Ren W;Yin J;Hu J;Duan J;Liu G;Tan B;Xiong X;Oso AO;Adeola O;Yao K;Yin Y;Li T
脱氧雪腐镰刀菌烯醇(DON)因摄入受污染的谷类产品而对人和猪产生多种毒理作用。本研究旨在探讨日粮中添加谷氨酸对DON攻击仔猪的保护作用。将20头28日龄断奶仔猪随机分为4组，每组5头：1)基础日粮，阴性对照(NC)；2)基础日粮+4 mg/kg DON(DON)；3)基础日粮+2%(g/g)谷氨酸(GLU)；4)基础日粮+4 mg/kg DON+2%谷氨酸(DG)。在7天的适应期之后是30天的治疗。采用核磁共振技术分析代谢产物，测定血浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、半胱氨酸天冬氨酸氨基转移酶-3(Caspase-3)活性及上皮细胞增殖情况。结果表明，DON处理组仔猪的低密度脂蛋白、丙氨酸、精氨酸、醋酸盐、糖蛋白、三甲胺-N-氧化物(TMAO)、甘氨酸、乳酸和尿素含量以及谷氨酸/肌酐比值均高于NC处理组，而高密度脂蛋白、脯氨酸、柠檬酸、胆碱、不饱和脂和富马酸含量低于NC处理组(P<；0.05)。与DON处理组相比，添加谷氨酸提高了血浆中脯氨酸、柠檬酸、肌酐、不饱和脂类和富马酸的浓度，降低了丙氨酸、糖蛋白、TMAO、甘氨酸和乳酸的浓度以及谷氨酸/肌酐的比值(P<；0.05)。在DON的基础上添加谷氨酸，可提高空肠和回肠组织中超氧化物歧化酶、谷胱甘肽过氧化物酶活性和增殖细胞核抗原标记指数(P<；0.05)。这些新的发现表明，谷氨酸具有修复氧化应激相关的损伤以及DON引起的能量和氨基酸代谢紊乱的潜力。
Deoxynivalenol (DON) has various toxicological effects in humans and pigs that result from the ingestion of contaminated cereal products. This study was conducted to investigate the protective effects of dietary supplementation with glutamic acid on piglets challenged with DON. A total of 20 piglets weaned at 28 d of age were randomly assigned to receive 1 of 4 treatments (5 piglets/treatment): 1) basal diet, negative control (NC); 2) basal diet +4 mg/kg DON (DON); 3) basal diet +2% (g/g) glutamic acid (GLU); 4) basal diet +4 mg/kg DON +2% glutamic acid (DG). A 7-d adaptation period was followed by 30 days of treatment. A metabolite analysis using nuclear magnetic resonance spectroscopy (1H-NMR)-based metabolomic technology and the determination of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities for plasma, as well as the activity of Caspase-3 and the proliferation of epithelial cells were conducted. The results showed that contents of low-density lipoprotein, alanine, arginine, acetate, glycoprotein, trimethylamine-N-oxide (TMAO), glycine, lactate, and urea, as well as the glutamate/creatinine ratio were higher but high-density lipoprotein, proline, citrate, choline, unsaturated lipids and fumarate were lower in piglets of DON treatment than that of NC treatment (P<0.05). Compared with DON treatment, dietary supplementation with glutamic acid increased the plasma concentrations of proline, citrate, creatinine, unsaturated lipids, and fumarate, and decreased the concentrations of alanine, glycoprotein, TMAO, glycine, and lactate, as well as the glutamate/creatinine ratio (P<0.05). Addition glutamic acid to DON treatment increased the plasma activities of SOD and GSH-Px and the proliferating cell nuclear antigen (PCNA) labeling indexes for the jejunum and ileum (P<0.05). These novel findings indicate that glutamic acid has the potential to repair the injuries associated with oxidative stress as well as the disturbances of energy and amino acid metabolism induced by DON.
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影响因子：
3.5
作者：
Boutry, Claire;Matsumoto, Hideki;Blachier, Francois
通讯作者：
Blachier, Francois
生长猪对日粮补充 L-精氨酸反应的代谢组学分析
影响因子：
3.5
作者：
He, Qinghua;Kong, Xiangfeng;Wu, Yongning
通讯作者：
Wu, Yongning
DOI：
10.1080/19440040903571747
发表时间：
2010-01-01
影响因子：
2.9
作者：
Awad, Wageha A.;Ghareeb, Khaled;Zentek, Juergen
通讯作者：
Zentek, Juergen
影响因子：
2.8
作者：
Dinu, Diana;Bodea, Gabriela Oana;Dinischiotu, Anca
通讯作者：
Dinischiotu, Anca
影响因子：
3.5
作者：
Diesing, Anne-Kathrin;Nossol, Constanze;Kahlert, Stefan
通讯作者：
Kahlert, Stefan