Conformational changes in DNA upon ligand binding monitored by circular dichroism.
Conformational changes in DNA upon ligand binding monitored by circular dichroism.
复制标题
通过圆二色性监测的配体结合后，DNA的构象变化。
DOI：
10.3390/ijms13033394
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发表时间：
2012
影响因子：
5.6
通讯作者：
Hou MH
中科院分区：
文献类型：
--
作者：
Chang YM;Chen CK;Hou MH
圆二色谱(CD)是一种测量左、右圆偏振光吸收差异的光学技术。这项技术被广泛应用于核酸结构的研究，在过去的几十年里，利用它来监测DNA的构象多态得到了极大的发展。DNA在与不同化合物结合的过程中，可能会发生构象变化，形成B型、A型、Z型、四联体、三联体等结构。本文综述了最近不同配体诱导DNA构象变化的圆二色谱研究，包括金黄色家族药物的金属衍生物、放线菌素D、新霉素、顺铂和多胺。显然，与其他技术相比，CD光谱学是非常敏感的，而且相对便宜。这些研究表明，CD光谱是监测药物结合引起的DNA构象变化的一种强有力的技术，也显示了它作为未来药物筛选平台的潜力。
Circular dichroism (CD) spectroscopy is an optical technique that measures the difference in the absorption of left and right circularly polarized light. This technique has been widely employed in the studies of nucleic acids structures and the use of it to monitor conformational polymorphism of DNA has grown tremendously in the past few decades. DNA may undergo conformational changes to B-form, A-form, Z-form, quadruplexes, triplexes and other structures as a result of the binding process to different compounds. Here we review the recent CD spectroscopic studies of the induction of DNA conformational changes by different ligands, which includes metal derivative complex of aureolic family drugs, actinomycin D, neomycin, cisplatin, and polyamine. It is clear that CD spectroscopy is extremely sensitive and relatively inexpensive, as compared with other techniques. These studies show that CD spectroscopy is a powerful technique to monitor DNA conformational changes resulting from drug binding and also shows its potential to be a drug-screening platform in the future.
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影响因子：
64.8
作者：
ALEXEEV, DG;LIPANOV, AA;SKURATOVSKII, IY
通讯作者：
SKURATOVSKII, IY
影响因子：
2.9
作者：
Gyi, JI;Conn, GL;Brown, T
通讯作者：
Brown, T
影响因子：
3.5
作者：
AIVASASHVILLI, VA;BEABEALASHVILLI, RS
通讯作者：
BEABEALASHVILLI, RS
影响因子：
2.9
作者：
CHEN, FM
通讯作者：
CHEN, FM
影响因子：
14.9
作者：
FRUGIER, M;FLORENTZ, C;GIEGE, R
通讯作者：
GIEGE, R