Conditional Cell Reprogramming and Air-Liquid Interface Modeling Life Cycle of Oncogenic Viruses (HPV and EBV) in Epithelial Cells and Virus-Associated Human Carcinomas.
Conditional Cell Reprogramming and Air-Liquid Interface Modeling Life Cycle of Oncogenic Viruses (HPV and EBV) in Epithelial Cells and Virus-Associated Human Carcinomas.
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DOI：
10.3390/v15061388
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发表时间：
2023-06-17
期刊：
影响因子：
--
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Rani AQ;Nurmemet D;Liffick J;Khan A;Mitchell D;Li J;Zhao B;Liu X
几种致癌病毒与大约20%的人类癌症有关。实验模型对于研究致癌病毒的致病性和生物学特性及其在肿瘤发生中的潜在机制至关重要。目前的细胞模型具有相当大的局限性，例如：它们的低产量，遗传和表观遗传修饰，以及在长繁殖期间肿瘤异质性的降低。癌细胞系是有限的，不适合研究病毒的生命周期，例如，HPV和EBV的自然病毒生命周期，其在上皮细胞中的持久性和潜伏期知之甚少，因为这些过程与上皮分化高度相关。因此，迫切需要可靠的人体生理细胞模型来研究病毒的生命周期和癌症的发生。条件性细胞重编程（CCR）是一种快速、稳定的细胞培养系统，可以从微创或非侵入性标本中建立细胞，并在长期培养过程中保留其谱系功能。这些CR细胞保留了它们在气液界面（ALI）分化的能力。在这里，我们概括了CR和ALI方法在模拟宿主-病毒相互作用和病毒介导的肿瘤发生中的应用。
Several oncogenic viruses are associated with approximately 20% of human cancers. Experimental models are crucial for studying the pathogenicity and biological aspects of oncogenic viruses and their potential mechanisms in tumorigenesis. Current cell models have considerable limitations such as: their low yield, genetic and epigenetic modification, and reduction in tumor heterogeneity during long propagation. Cancer cell lines are limited and not appropriate for studying the viral life cycle, for example, natural viral life cycles of HPV and EBV, and their persistence and latency in epithelial cells are poorly understood, since these processes are highly related to epithelial differentiation. Therefore, there is an urgent need of reliable human physiological cell models to study viral life cycle and cancer initiation. Conditional cell reprogramming (CCR) is a rapid and robust cell culture system, where the cells can be established from minimally invasive or noninvasive specimens and their lineage functions preserved during the long-term culture. These CR cells retain their ability to differentiate at air–liquid interface (ALI). Here, we recapitulated the applications of CR and ALI approaches in modeling host–virus interactions and viral-mediated tumorigenesis.
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DOI：
10.2147/dddt.s49584
发表时间：
2014
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Cekanova M;Rathore K
通讯作者：
Rathore K
影响因子：
4.3
作者：
Xing, Biyuan;Pu, Congli;Chen, Yunshang;Sheng, Yuhan;Zhang, Baofang;Cui, Jie;Wu, Gang;Zhao, Yingchao
通讯作者：
Zhao, Yingchao
影响因子：
--
作者：
Alam, M. Shabbir;Ali, Asgar;Rizvi, M. Moshahid Alam
通讯作者：
Rizvi, M. Moshahid Alam
影响因子：
5.4
作者：
Bienkowska-Haba, Malgorzata;Zwolinska, Katarzyna;Sapp, Martin
通讯作者：
Sapp, Martin
影响因子：
4.6
作者：
Chen C;Choudhury S;Wangsa D;Lescott CJ;Wilkins DJ;Sripadhan P;Liu X;Wangsa D;Ried T;Moskaluk C;Wick MJ;Glasgow E;Schlegel R;Agarwal S
通讯作者：
Agarwal S