Incongruence between nuclear and chloroplast DNA phylogenies in pedicularis section Cyathophora (Orobanchaceae).

Incongruence between nuclear and chloroplast DNA phylogenies in pedicularis section Cyathophora (Orobanchaceae).
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小儿cyathophora(Orobanchaceae)中的核成形细胞系统发育不一致。

DOI:
10.1371/journal.pone.0074828
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发表时间:
2013
期刊:
影响因子:
3.7
通讯作者:
Wang H
Wang H
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Yu WB;Huang PH;Li DZ;Wang H

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马先蒿科 Cyathophora 是一个单系类群,其特征是每个节处有穿孔叶和/或苞片基部。本节包括四个系列,分别对应马先蒿的四种一般花冠类型,即无齿花冠、有齿花冠、喙花冠和长管花冠。在这项研究中,我们的目标是重建 Cyathophora 部分的全面系统发育,并比较核数据集和叶绿体数据集之间的系统发育不一致。对 67 份属于马先蒿科的材料和 9 份属于马先蒿属的材料进行了取样,并对 1 个核基因 (nrITS) 和 4 个叶绿体基因 (matK、rbcL、trnH-psbA 和 trnL-F) 进行了测序。系统发育分析表明,从 nrITS 和串联叶绿体数据集推断出的拓扑和网络不一致,并且 nrITS 系统发育和网络在一定程度上与基于形态的分类学一致。四川两个样本的叶绿体基因组。 cyathophylloides (E4 和 E5) 可能显示来自 P 的祖先的基因渗入。叶藻。 nrITS 数据集和串联叶绿体数据集都无法充分解析 Reges 系列中物种之间的关系;这很可能是由于不完整的谱系排序和/或基因渗入/杂交造成的。 nrITS系统发育表明,无喙(有齿和无牙)和喙帽状帽藻可能是在Cyathophora组内独立进化的,而叶绿体系统发育表明,长花冠管和带喙帽状帽帽是从短花冠管衍生而来。
Pedicularis section Cyathophora is a monophyletic group characterized by perfoliate leaf and/or bract bases at each node. This section comprises four series, corresponding to four general corolla types of Pedicularis , i.e. toothless, toothed, beaked and long-tubed corollas. In this study, we aim to reconstruct a comprehensive phylogeny of section Cyathophora , and compare phylogenetic incongruence between nuclear and chloroplast datasets. Sixty-seven accessions belonging to section Cyathophora and 9 species for other Pedicularis were sampled, and one nuclear gene (nrITS) and four chloroplast genes (matK, rbcL, trnH-psbA and trnL-F) were sequenced. Phylogenetic analyses show that the topologies and networks inferred from nrITS and the concatenated chloroplast datasets were incongruent, and the nrITS phylogenies and network agreed with the morphology-based taxonomy to some degree. The chloroplast genome of two Sichuan samples of P . cyathophylloides (E4 and E5) may show introgression from an ancestor of P . cyathophylla . Neither the nrITS dataset nor the concatenated chloroplast dataset were able to adequately resolve relationships among species in the series Reges; this is most likely due to incomplete lineage sorting and/or introgression/hybridization. The nrITS phylogeny indicates the beakless (toothed and toothless) and beaked galeas may have evolved independently within section Cyathophora , and the chloroplast phylogeny reveals that the long corolla tube with beaked galea is derived from the short one.
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