MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4.
MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4.
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DOI：
10.1080/21655979.2021.1871817
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发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Jie Y
中科院分区：
文献类型：
--
作者：
Cai L;Ye L;Hu X;He W;Zhuang D;Guo Q;Shu K;Jie Y
已有研究报道miR-330- 3 p参与多种肿瘤的发生发展，但其在卵巢癌（OC）中的作用尚不清楚。本研究旨在探讨miR-330- 3 p在OC中的表达模式及其功能。采用RT-qPCR检测miR-330- 3 p在OC组织和细胞系中的表达水平。CCK-8法检测OC细胞的增殖能力，transwell法检测OC细胞的迁移能力和侵袭能力。采用生物信息学分析和荧光素酶报告基因分析方法分析miR-330- 3 p与RIPK 4的靶向结合位点。结果显示，miR-330- 3 p在OC组织和细胞系中表达显著下调。过表达miR-330- 3 p可抑制OC细胞的增殖、迁移和侵袭。从机制上讲，双荧光素酶报告的测定显示RIPK 4是miR-330- 3 p的靶基因。此外，拯救实验显示miR-330- 3 p通过靶向RIPK 4抑制OC细胞的增殖、迁移和侵袭。总之，我们的研究结果表明，miR-330- 3 p通过靶向RIPK 4抑制OC的进展。我们的研究结果提示miR-330- 3 p/RIPK 4轴可能成为OC治疗的新靶点。
Previous studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330-3p in OC tissues and cell lines was detected using RT-qPCR. The proliferation, migration and invasion of OC cells were detected using CCK-8 assay and transwell assay, respectively. Bioinformatics analysis and luciferase reporter assay were used to analyze the targeted binding site of miR-330-3p and RIPK4. The results showed that miR-330-3p was significantly downregulated in OC tissues and cell lines. Overexpression of miR-330-3p inhibited the proliferation, migration and invasion of OC cells. Mechanistically, a dual-luciferase reported assay showed that RIPK4 is a target gene of miR-330-3p. Furthermore, rescue experiments revealed that miR-330-3p suppressed the proliferation, migration and invasion of OC cells by targeting RIPK4. In summary, our findings indicated that miR-330-3p suppressed the progression of OC by targeting RIPK4. Our results indicated that miR-330-3p/RIPK4 axis might act as a novel therapeutic target for OC treatment.
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影响因子：
3.3
作者：
Kage H;Borok Z
通讯作者：
Borok Z
影响因子：
14.9
作者：
Karagkouni D;Paraskevopoulou MD;Chatzopoulos S;Vlachos IS;Tastsoglou S;Kanellos I;Papadimitriou D;Kavakiotis I;Maniou S;Skoufos G;Vergoulis T;Dalamagas T;Hatzigeorgiou AG
通讯作者：
Hatzigeorgiou AG
DOI：
10.1007/978-1-61779-037-9_3
发表时间：
2011-01-01
期刊：
ANTIVIRAL RNAI: CONCEPTS, METHODS, AND APPLICATIONS
影响因子：
--
作者：
Plaisance-Bonstaff, Karlie;Renne, Rolf
通讯作者：
Renne, Rolf
影响因子：
2.9
作者：
Jin, Zhe;Jia, Baoxing;Liu, Yahui
通讯作者：
Liu, Yahui
影响因子：
4.8
作者：
Bähr, C;Rohwer, A;Gschwendt, M
通讯作者：
Gschwendt, M