Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (Bombyx mori).
Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (Bombyx mori).
复制标题
比较蛋白质组学分析为家蚕（Bombyx mori）热诱导孤雌生殖的分子基础提供了新的见解
作者：
单性生殖是卵母细胞不经受精而进入发育的一种生殖方式，在昆虫中很常见，在进化、社会性和生态学中起着重要作用。然而，昆虫孤雌生殖的分子机制仍有待进一步研究。家蚕是一种重要的经济昆虫。利用热刺激诱导家蚕离体孤雌生殖作为一种性别控制方法，已在家蚕育种中得到广泛应用。深入了解孤雌生殖及其调控机制对蚕业生产具有重要意义。同时，家蚕作为鳞翅目动物模型，为孤雌生殖发育研究提供了良好的材料。本研究采用基于iTRAQ的定量蛋白质组学方法，比较了完全孤雌生殖系（PL）及其亲本双殖系（AL）的蛋白质表达谱。在PL中，在热诱导前鉴定出274个丰度增加的蛋白质和211个丰度降低的蛋白质，在热诱导后鉴定出97个丰度增加的蛋白质和187个丰度降低的蛋白质。功能分析揭示了两种不同生殖模式的分子特征，以及参与减数分裂过程对热刺激的反应的蛋白质和途径。这些结果为家蚕孤雌生殖的分子机制提供了新的认识。摘要家蚕热刺激孤雌生殖是蚕业生产中的一项重要技术。然而，其背后的分子机制在很大程度上仍然未知。我们已经创建了一个完全的孤雌生殖线（PL）超过85%的发生率和80%的孵化率，通过热水处理和遗传选择，而双亲两性系（AL）进行相同的处理时，色素沉着率低于30%，孵化率低于1%。在这里，同量异序标签的相对和绝对定量（iTRAQ）为基础的分析被用来调查的关键蛋白和途径与家蚕孤雌生殖。我们发现了PL中未受精卵的独特蛋白质组学特征。总共，274个增加的丰度蛋白质和211个减少的丰度蛋白质被确定相对于热诱导前的AL。功能分析显示PL的翻译和代谢水平增加。热诱导后，鉴定出97个丰度增加的蛋白和187个丰度降低的蛋白。应激反应相关蛋白的增加和能量代谢的减少表明PL比AL更有效地缓冲热应激。细胞周期相关蛋白，包括组蛋白和纺锤体相关蛋白在PL中减少，表明这种减少在无减数分裂孤雌生殖过程中的重要作用。
Simple Summary Parthenogenesis is a reproductive mode by which an oocyte goes into development without fertilization, which is common in insects and plays an important role in evolution, sociality, and ecology. However, the molecular mechanism of parthenogenesis in insects remains to be expanded. The silkworm Bombyx mori is an economically important insect. The artificial parthenogenesis induced in vitro using thermal stimuli has been well used in silkworm breeding as a sex control method. A better understanding of parthenogenesis and its regulatory mechanisms could benefit sericultural production. Meanwhile, silkworm provides a good material for parthenogenesis development research as a lepidopteran model. In this study, iTRAQ-based quantitative proteomics were carried out to compare the protein expression profile between a fully parthenogenetic line (PL) and its parent amphigenetic line (AL). We identified 274 increased abundance proteins and 211 decreased abundance proteins before thermal induction, and 97 increased abundance proteins and 187 decreased abundance proteins after thermal induction in PL. Function analysis uncovered the molecular signatures underlying the two different reproductive modes, and the proteins and pathways involved in a meiosis process response to thermal stimuli. The results provide new insights into the molecular mechanism underlying silkworm parthenogenesis. Abstract Artificial parthenogenetic induction via thermal stimuli in silkworm is an important technique that has been used in sericultural production. However, the molecular mechanism underlying it remains largely unknown. We have created a fully parthenogenetic line (PL) with more than 85% occurrence and 80% hatching rate via hot water treatment and genetic selection, while the parent amphigenetic line (AL) has less than 30% pigmentation rate and less than 1% hatching rate when undergoing the same treatment. Here, isobaric tags for relative and absolute quantitation (iTRAQ)-based analysis were used to investigate the key proteins and pathways associated with silkworm parthenogenesis. We uncovered the unique proteomic features of unfertilized eggs in PL. In total, 274 increased abundance proteins and 211 decreased abundance proteins were identified relative to AL before thermal induction. Function analysis displayed an increased level of translation and metabolism in PL. After thermal induction, 97 increased abundance proteins and 187 decreased abundance proteins were identified. An increase in stress response-related proteins and decrease in energy metabolism suggested that PL has a more effective response to buffer the thermal stress than AL. Cell cycle-related proteins, including histones, and spindle-related proteins were decreased in PL, indicating an important role of this decrease in the process of ameiotic parthenogenesis.
登录
查看更多内容
DOI：
10.1016/j.cub.2017.08.031
发表时间：
2017-10-09
期刊：
Current biology : CB
影响因子：
--
作者：
Gimpel P;Lee YL;Sobota RM;Calvi A;Koullourou V;Patel R;Mamchaoui K;Nédélec F;Shackleton S;Schmoranzer J;Burke B;Cadot B;Gomes ER
通讯作者：
Gomes ER
影响因子：
4
作者：
Dedryver, Charles-Antoine;Bonhomme, Joel;Simon, Jean-Christophe
通讯作者：
Simon, Jean-Christophe
DOI：
10.1007/978-1-0716-1979-7_2
发表时间：
2022-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Crasta, Daphne Norma;Adiga, Satish Kumar;Kalthur, Guruprasad
通讯作者：
Kalthur, Guruprasad
影响因子：
56.9
作者：
DOANE, WW
通讯作者：
DOANE, WW
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta