Vascular endothelial growth factor, carbonic anhydrase 9, and angiogenin as urinary biomarkers for bladder cancer detection.
Vascular endothelial growth factor, carbonic anhydrase 9, and angiogenin as urinary biomarkers for bladder cancer detection.
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DOI：
10.1016/j.urology.2012.01.016
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发表时间：
2012-05
期刊：
影响因子：
2.1
通讯作者：
Rosser CJ
中科院分区：
文献类型：
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作者：
Urquidi V;Goodison S;Kim J;Chang M;Dai Y;Rosser CJ
研究尿中血管内皮生长因子（VEGF）、碳酸酐酶9（CA 9）和血管生成素水平升高是否与BCa相关。这是一项病例对照研究，分析了127例患者的排尿：对照受试者（n = 63）和肿瘤受试者（n = 64）。采用酶联免疫吸附试验（ELISA）检测尿VEGF、CA 9、血管生成素和BTA浓度。我们使用受试者工作特征曲线的曲线下面积（AUC）来确定VEGF、CA 9和血管生成素检测排尿样本中BCa的能力。还将数据与商业化的基于ELISA的BCa检测测定（BTA-Trak ®）进行比较。计算敏感性、特异性、阳性和阴性预测值。BCa组尿VEGF、CA 9、angiogenin和BTA浓度显著升高。VEGF是最准确的尿液生物标志物（AUC：0.886; 95%置信区间[CI]：0.8301-0.9418）。此外，多变量回归分析强调VEGF（OR：5.90; 95%CI：2.60-13.40，p < 0.0001）为自变量。VEGF的敏感性和特异性（敏感性，83%和特异性，87%）优于BTA（敏感性，80%和特异性，84%）。VEGF可能是一个有价值的除了尿液样本分析检测BCa。需要更大规模的前瞻性研究来确定尿VEGF和血管生成素作为生物标志物在BCa患者非侵入性评估中的临床效用。
To investigate whether elevated urinary levels of vascular endothelial growth factor (VEGF), carbonic anhydrase 9 (CA9) and angiogenin are associated with BCa. This is a case-control study in which voided urines from 127 patients: control subjects (n = 63) and tumor bearing subjects (n = 64) were analyzed. The urinary concentrations of VEGF, CA9, angiogenin and BTA were assessed by enzyme-linked immunosorbent assay (ELISA). We used the area under the curve (AUC) of receiver operating characteristic curves to determine the ability of VEGF, CA9, and angiogenin to detect BCa in voided urine samples. Data were also compared to a commercial ELISA-based BCa detection assay (BTA-Trak©). Sensitivity, specificity, positive and negative predictive values were calculated. Urinary concentrations of VEGF, CA9, angiogenin and BTA were significantly elevated in BCa. VEGF was the most accurate urinary biomarker (AUC: 0.886; 95% confidence interval [CI]: 0.8301–0.9418). Furthermore, multivariate regression analysis highlighted VEGF (OR: 5.90; 95% CI: 2.60–13.40, p < 0.0001) as an independent variable. The sensitivities and specificities for VEGF (sensitivity, 83% and specificity, 87%) outperformed BTA (sensitivity, 80% and specificity, 84%). VEGF may be a valuable addition to voided urine sample analysis for the detection of BCa. Larger, prospective studies are needed to determine the clinical utility of urinary VEGF and angiogenin as biomarkers in the non-invasive evaluation of BCa patients.
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DOI：
10.1158/1055-9965.epi-08-1002
发表时间：
2009-02
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
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作者：
Rosser CJ;Liu L;Sun Y;Villicana P;McCullers M;Porvasnik S;Young PR;Parker AS;Goodison S
通讯作者：
Goodison S
影响因子：
6.6
作者：
Eissa, Sanaa;Swellam, Menha;El Ahmady, Omar
通讯作者：
El Ahmady, Omar
影响因子：
2.6
作者：
Bian, Wei;Xu, Zhishun
通讯作者：
Xu, Zhishun
关键评估用于分类或预测的生物标志物的准确性：研究设计标准。
影响因子：
10.3
作者：
Pepe, Margaret S.;Feng, Ziding;Janes, Holly;Bossuyt, Patrick M.;Potter, John D.
通讯作者：
Potter, John D.
影响因子：
8.8
作者：
通讯作者：
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