Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections.
Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections.
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DOI：
10.1016/j.xpro.2021.100830
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Mourkioti F
中科院分区：
文献类型：
--
作者：
Tichy ED;Mourkioti F
骨骼肌干细胞（MuSCs）是肌肉中一种罕见的细胞群，对其端粒长度的测量为疾病状态下的细胞功能障碍提供了见解。在这里，我们描述了一种方案（冷冻肌肉定量荧光原位杂交），使用骨骼肌冷冻来评估musc在其原生环境中的端粒长度。使用免费软件，端粒长度测量在单细胞水平上进行评估。我们还提供了以几种方式执行数据分析的方法。有关使用和执行本协议的完整详情，请参阅。CRYO MuQ-FISH可以测量常驻骨骼肌干细胞的端粒长度，该方案是档案样品的理想选择，端粒长度定量可以归一化为DAPI或着丝粒强度端粒输出包括每核的总强度和平均强度，骨骼肌干细胞（MuSCs）是肌肉中罕见的细胞群，端粒长度的测量提供了对疾病条件下细胞功能障碍的见解。在这里，我们描述了一种方案（冷冻肌肉定量荧光原位杂交），使用骨骼肌冷冻来评估musc在其原生环境中的端粒长度。使用免费软件，端粒长度测量在单细胞水平上进行评估。我们还提供了以几种方式执行数据分析的方法。
Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situhybridization) using skeletal muscle cryosections for assessments of telomere length in MuSCs, in their native environment. Using a free software, telomere length measurements are assessed on a single-cell level. We also provide methodology to perform data analyses in several ways. For complete details on the use and execution of this protocol, please refer to. CRYO MuQ-FISH can measure telomere length on resident skeletal muscle stem cells This protocol is ideal for archival samples Telomere length quantitation can be normalized to DAPI or centromere intensity Telomere outputs include both total sum intensity and mean intensity per nucleus Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situhybridization) using skeletal muscle cryosections for assessments of telomere length in MuSCs, in their native environment. Using a free software, telomere length measurements are assessed on a single-cell level. We also provide methodology to perform data analyses in several ways.
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影响因子：
8.8
作者：
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通讯作者：
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通讯作者：
Mourkioti F