Bioelectrochemical immunoassay of polychlorinated biphenyl.
Bioelectrochemical immunoassay of polychlorinated biphenyl.
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多氯联苯的生物电化学免疫分析。
DOI：
10.1016/j.aca.2008.01.080
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发表时间：
2008
影响因子：
6.2
通讯作者：
Lin,Yuehe
中科院分区：
文献类型：
--
作者：
Lin,Ying-Ying;Liu,Guodong;Wai,CM;Lin,Yuehe
建立了一种简单、快速、高灵敏度的基于磁珠和一次性丝网印刷电极的多氯联苯（PCBs）生物电化学免疫分析方法。该生物测定法的原理是基于直接竞争酶联免疫吸附测定法，使用多氯联苯抗体包被的MB和辣根过氧化物酶（HRP）标记的多氯联苯（HRP-PCB）。在免疫反应过程中使用磁性处理平台混合和摇动样品，并在PCB-抗体包被的MB、PCB分析物和HRP-PCB之间的液相竞争免疫反应后分离游离和未结合的试剂。在一个完整的免疫测定，HRP示踪剂连接到MB转移到底物溶液含有邻氨基苯酚和过氧化氢的电化学检测。不同的参数，包括HRP-PCB结合物的量，免疫反应时间，和底物的浓度，决定了免疫分析的分析性能进行了详细的研究和优化。在最佳实验条件下，检出限为10 pgmL-1。用未处理过的河水对生物电化学免疫分析法进行了评价，并用市售的PCBs酶联免疫吸附测定试剂盒对结果进行了验证，表明该方法简便、灵敏，可用于分散环境监测和痕量PCBs的检测。
A simple, rapid, and highly sensitive bioelectrochemical immunoassay method based on magnetic beads (MBs) and disposable screen-printed electrodes (SPE) has been developed to detect polychlorinated biphenyls (PCBs). The principle of this bioassay is based on a direct competitive enzyme-linked immunosorbent assay using PCB-antibody-coated MBs and horseradish peroxidase (HRP)-labeled PCB (HRP–PCB). A magnetic process platform was used to mix and shake the samples during the immunoreactions and to separate free and unbound reagents after the liquid-phase competitive immunoreactions among PCB-antibody-coated MBs, PCB analyte, and HRP–PCB. After a complete immunoassay, the HRP tracers attached to MBs were transferred to a substrate solution containing o-aminophenol and hydrogen peroxide for electrochemical detection. The different parameters, including the amount of HRP–PCB conjugates, immunoreaction time, and the concentration of substrate that governs the analytical performance of the immunoassay have been studied in detail and optimized. The detection limit of 10pgmL−1was obtained under optimum experimental conditions. The performance of this bioelectrochemical immunoassay was successfully evaluated with untreated river water spiked with PCBs, and the results were validated by commercial PCB enzyme-linked immunosorbent assay kit, indicating that this convenient and sensitive technique offers great promise for decentralized environmental application and trace PCBs monitoring.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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