Endometrial stromal cells of women with recurrent miscarriage fail to discriminate between high- and low-quality human embryos.
Endometrial stromal cells of women with recurrent miscarriage fail to discriminate between high- and low-quality human embryos.
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DOI：
10.1371/journal.pone.0041424
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Macklon NS
中科院分区：
文献类型：
--
作者：
Weimar CH;Kavelaars A;Brosens JJ;Gellersen B;de Vreeden-Elbertse JM;Heijnen CJ;Macklon NS
复发性流产（RM）的病因在很大程度上仍然无法解释。患有RM的女性比正常生育的女性有更短的怀孕时间间隔，这可能是由于更频繁地植入非存活胚胎。我们假设RM患者的子宫内膜间质细胞（H-EnSC）在高质量和低质量人类胚胎之间的区分效果较差，并且比正常生育妇女的H-EnSC更容易向滋养层球体迁移。从6名RM女性和6名生育对照的子宫内膜活检中产生单层的蜕膜化H-EnSC。建立无细胞迁移区，并在18小时后分析高质量（第5天胚泡，n = 13）、低质量（第5天胚泡，具有三个原核或发育不全的胚胎，n = 12）或AC-1 M88滋养层细胞系球状体的存在对H-ESC迁移活性的影响。 在没有球体或胚胎的情况下，来自可育或RM女性的H-EnSC的迁移是相似的。在该区域中存在低质量胚胎的情况下，与不存在胚胎的基础迁移相比（P<0.05），以及与存在高质量胚胎的迁移相比（p<0.01），对照女性的H-EnSC迁移受到抑制。有趣的是，RM女性的迁移反应H-EnSC在高质量和低质量胚胎之间没有差异。此外，在球体存在下，与对照的H-EnSC相比，它们的迁移增强（p<0.001）。可生育妇女的H-EnSC区分高质量和低质量的胚胎，而RM妇女的H-EnSC不能做到这一点。RM妇女的H-EnSC对滋养层球体具有更高的迁移反应。未来的研究将集中在低质量胚胎抑制H-EnSC迁移的机制，以及这在RM女性中是如何解除管制的。
The aetiology of recurrent miscarriage (RM) remains largely unexplained. Women with RM have a shorter time to pregnancy interval than normally fertile women, which may be due to more frequent implantation of non-viable embryos. We hypothesized that human endometrial stromal cells (H-EnSCs) of women with RM discriminate less effectively between high-and low-quality human embryos and migrate more readily towards trophoblast spheroids than H-EnSCs of normally fertile women. Monolayers of decidualized H-EnSCs were generated from endometrial biopsies of 6 women with RM and 6 fertile controls. Cell-free migration zones were created and the effect of the presence of a high-quality (day 5 blastocyst, n = 13), a low-quality (day 5 blastocyst with three pronuclei or underdeveloped embryo, n = 12) or AC-1M88 trophoblast cell line spheroid on H-ESC migratory activity was analyzed after 18 hours. In the absence of a spheroid or embryo, migration of H-EnSCs from fertile or RM women was similar. In the presence of a low-quality embryo in the zone, the migration of H-EnSCs of control women was inhibited compared to the basal migration in the absence of an embryo (P<0.05) and compared to the migration in the presence of high-quality embryo (p<0.01). Interestingly, the migratory response H-EnSCs of women with RM did not differ between high- and low-quality embryos. Furthermore, in the presence of a spheroid their migration was enhanced compared to the H-EnSCs of controls (p<0.001). H-EnSCs of fertile women discriminate between high- and low-quality embryos whereas H-EnSCs of women with RM fail to do so. H-EnSCs of RM women have a higher migratory response to trophoblast spheroids. Future studies will focus on the mechanisms by which low-quality embryos inhibit the migration of H-EnSCs and how this is deregulated in women with RM.
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影响因子：
6.1
作者：
Leese, Henry J.;Sturmey, Roger G.;McEvoy, Tom G.
通讯作者：
McEvoy, Tom G.
影响因子：
3.7
作者：
Teklenburg G;Salker M;Molokhia M;Lavery S;Trew G;Aojanepong T;Mardon HJ;Lokugamage AU;Rai R;Landles C;Roelen BA;Quenby S;Kuijk EW;Kavelaars A;Heijnen CJ;Regan L;Brosens JJ;Macklon NS
通讯作者：
Macklon NS
影响因子：
3.8
作者：
Redman, C. W. G.;Sargent, I. L.
通讯作者：
Sargent, I. L.
DOI：
10.1073/pnas.0806219105
发表时间：
2008-10-21
影响因子：
11.1
作者：
Grewal, Seema;Carver, Janet G.;Mardon, Helen J.
通讯作者：
Mardon, Helen J.
影响因子：
6.1
作者：
Stokes, Paula J.;Hawkhead, Judith A.;Houghton, Franchesca D.
通讯作者：
Houghton, Franchesca D.