Transcription in the maintenance of centromere chromatin identity.
Transcription in the maintenance of centromere chromatin identity.
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DOI：
10.1093/nar/gks921
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发表时间：
2012-12
影响因子：
14.9
通讯作者：
Wong LH
中科院分区：
文献类型：
--
作者：
Chan FL;Wong LH
最近的证据表明，转录是允许的，通过据称抑制性着丝粒结构域，这种转录活性是功能性的后果。研究得最好的例子是在酵母中产生臂间异染色质组装所需的siRNA时，臂间DNA重复序列的转录。然而，也已检测到来自臂间和着丝粒核心结构域的非siRNA转录物以细胞周期和细胞分化依赖的方式存在。在一些癌症中也检测到着丝粒转录物水平升高;然而，尚不清楚高水平的着丝粒转录物可能对疾病进展有多大贡献。最近的研究表明，在着丝粒处仔细调节组蛋白修饰和转录水平对于关键着丝粒蛋白的募集和CENP-A结构域的组装至关重要。在这里，我们比较了来自近着丝粒和着丝粒核心区域的各种转录物的转录动力学和功能。我们还提出了一个模型，在该模型中，染色质重塑活动的转录，并由此产生的成绩单，有助于协同永久着丝粒染色质的身份。
Recent evidence has shown that transcription is permissible through the purportedly repressive centromere domain, and that this transcriptional activity is of functional consequence. The best-studied example is transcription of the pericentric DNA repeats in the generation of siRNAs required for pericentric heterochromatin assembly in yeast. However, non-siRNA transcripts emanating from both pericentric and centromere core domains have also been detected in a cell cycle and cellular differentiation-dependent manner. Elevated levels of centromeric transcripts have also been detected in some cancers; however, it is still unclear how high levels of centromere transcripts may contribute towards disease progression. More recent studies have demonstrated that careful regulation of the histone modifications and transcription level at the centromere is vital for the recruitment of key centromere proteins and assembly of CENP-A domain. Here, we compare the transcriptional dynamics and function of various transcripts derived from pericentromeric and centromere core regions. We also propose a model in which the chromatin remodelling activity of transcription, and the resultant transcripts, contribute synergistically to perpetuate centromere chromatin identity.
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影响因子：
4.5
作者：
Chueh, Anderly C.;Northrop, Emma L.;Brettingham-Moore, Kate H.;Choo, K. H. Andy;Wong, Lee H.
通讯作者：
Wong, Lee H.
影响因子：
4.5
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Du Y;Topp CN;Dawe RK
通讯作者：
Dawe RK
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11.4
作者：
Djupedal, Ingela;Kos-Braun, Isabelle C.;Ekwall, Karl
通讯作者：
Ekwall, Karl
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16
作者：
Chen, ES;Saitoh, S;Takahashi, K
通讯作者：
Takahashi, K
影响因子：
56.9
作者：
Dion, Michael F.;Kaplan, Tommy;Rando, Oliver J.
通讯作者：
Rando, Oliver J.