Dynamically bonded layer-by-layer films for self-regulated insulin release
Dynamically bonded layer-by-layer films for self-regulated insulin release
复制标题
用于自我调节胰岛素释放的动态粘合逐层薄膜
DOI：
10.1039/c2jm33413e
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发表时间：
2012-07
影响因子：
--
通讯作者：
Zhang, Yongjun
中科院分区：
文献类型：
--
作者：
Zhang, Xi;Guan, Ying;Zhang, Yongjun
使用聚合物对之间的可逆/动态键作为驱动力的逐层组装膜本质上是动态的。在某些条件下，由于动态键的断裂，它们可能会分解。更重要的是，它们在平衡控制的条件下逐渐崩解，使它们适合于持续药物释放。在这里，从动态LbL膜的胰岛素释放被证明作为一个例子。膜由荧光标记的胰岛素-PVA（PVA：聚乙烯醇）缀合物和聚[丙烯酰胺-共-3-（丙烯酰胺基）-苯基硼酸]使用可逆共价键制造，即，苯基硼酸酯键，作为驱动力。膜制造之后进行紫外-可见光谱和荧光光谱。在所有情况下，观察到线性膜生长。膜的生长速率随着组装溶液的pH降低和离子强度的增加而增加。通过胰岛素与PVA的共价缀合实现了胰岛素到膜中的成功整合。当浸入水溶液中时，膜逐渐崩解，从而将胰岛素释放到培养基中。胰岛素释放速率随着介质的pH增加和离子强度降低而增加。更重要的是，它随着培养基中葡萄糖浓度的增加而增加。膜的葡萄糖响应归因于膜中的PBA（PBA：苯基硼酸）基团由于形成葡萄糖-PBA络合物而从中性形式转化为带负电荷的形式，从而增加膜崩解的速率。
Layer-by-layer assembled films using reversible/dynamic bonds between the polymer pairs as driving forces are dynamic in nature. They may disintegrate under certain conditions as a result of the breakage of the dynamic bonds. More importantly they disintegrate gradually under conditions of equilibrium control, making them suitable for sustained drug release. Here insulin release from dynamic LbL films was demonstrated as an example. The films were fabricated from a fluorescently-labeled insulin–PVA (PVA: polyvinyl alcohol) conjugate and poly[acrylamide-co-3-(acrylamido)-phenylboronic acid], using a reversible covalent bond, i.e., phenylboronate ester bond, as the driving force. Film fabrication was followed by UV-vis and fluorescence spectroscopy. In all cases linear film growth was observed. The film growth rate increases with decreasing pH and increasing ionic strength of the assembly solutions. Successful integration of insulin into the films was achieved by covalent conjugation of insulin with PVA. When immersed in an aqueous solution, the films disintegrate gradually, thus releasing insulin into the media. Insulin release rate increases with increasing pH and decreasing ionic strength of the media. More importantly, it increases with increasing glucose concentration in the media. The glucose response of the film was attributed to the conversion of PBA (PBA: phenylboronic acid) groups in the film from a neutral form to a negatively charged one as a result of the formation of glucose–PBA complexes, thus increasing the rate of the film disintegration.
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DOI：
10.1021/la200815t
发表时间：
2011-06-21
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Flessner RM;Jewell CM;Anderson DG;Lynn DM
通讯作者：
Lynn DM
影响因子：
14
作者：
Konno, Tomohiro;Ishihara, Kazuhiko
通讯作者：
Ishihara, Kazuhiko
影响因子：
6.2
作者：
Matsumoto, A;Yoshida, R;Kataoka, K
通讯作者：
Kataoka, K
影响因子：
14
作者：
Blacklock, Jenifer;Handa, Hitesh;Oupicky, David
通讯作者：
Oupicky, David
影响因子：
3.9
作者：
Yong Zhang and;W. Cao
通讯作者：
Yong Zhang and;W. Cao