Lack of tissue renewal in human adult Achilles tendon is revealed by nuclear bomb (14)C.
Lack of tissue renewal in human adult Achilles tendon is revealed by nuclear bomb (14)C.
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DOI：
10.1096/fj.12-225599
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Kjaer M
中科院分区：
文献类型：
--
作者：
Heinemeier KM;Schjerling P;Heinemeier J;Magnusson SP;Kjaer M
肌腱经常受伤，愈合不良。这是否是由缓慢的组织周转引起的尚不清楚，因为现有的数据提供了不同的肌腱蛋白半衰期估计，范围从2个月到200年。为了测定人肌腱组织的终身更新，我们使用了14 C弹-脉冲法。这种方法利用了1955-1963年核弹试验产生的大气中14 C水平的急剧增加，这反映在所有生物体中。用加速器质谱法（AMS）测量了28个跟腱核心法医样本和4个骨骼肌样本（供体出生年份1945-1983）中的14 C水平，并与已知的大气水平进行比较，以估计组织周转。我们发现跟腱组织保留了与组织采样前几十年大气水平相对应的14 C水平，这表明组织更新非常有限。肌腱的14 C浓度大致反映了在生命的第一个17年期间存在的大气水平，表明肌腱核心是在高度增长过程中形成的，此后基本上不再更新。相比之下，肌肉中的14 C水平表明持续的周转。我们的观察为理解肌腱功能和病理学提供了一个基本前提，并可能解释了肌腱组织再生能力差的原因。Heinemeier，K. M.，Schjerling，P.，Heinemeier，J.，Magnusson，S. P.，Kjaer，M.核弹14 C揭示了人类成年跟腱组织更新的缺乏。
Tendons are often injured and heal poorly. Whether this is caused by a slow tissue turnover is unknown, since existing data provide diverging estimates of tendon protein half-life that range from 2 mo to 200 yr. With the purpose of determining life-long turnover of human tendon tissue, we used the 14C bomb-pulse method. This method takes advantage of the dramatic increase in atmospheric levels of 14C, produced by nuclear bomb tests in 1955–1963, which is reflected in all living organisms. Levels of 14C were measured in 28 forensic samples of Achilles tendon core and 4 skeletal muscle samples (donor birth years 1945–1983) with accelerator mass spectrometry (AMS) and compared to known atmospheric levels to estimate tissue turnover. We found that Achilles tendon tissue retained levels of 14C corresponding to atmospheric levels several decades before tissue sampling, demonstrating a very limited tissue turnover. The tendon concentrations of 14C approximately reflected the atmospheric levels present during the first 17 yr of life, indicating that the tendon core is formed during height growth and is essentially not renewed thereafter. In contrast, 14C levels in muscle indicated continuous turnover. Our observation provides a fundamental premise for understanding tendon function and pathology, and likely explains the poor regenerative capacity of tendon tissue.—Heinemeier, K. M., Schjerling, P., Heinemeier, J., Magnusson, S. P., Kjaer, M. Lack of tissue renewal in human adult Achilles tendon is revealed by nuclear bomb 14C.
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影响因子：
5.5
作者：
Miller, BF;Olesen, JL;Rennie, MJ
通讯作者：
Rennie, MJ
影响因子：
2.9
作者：
Svensson, Rene B.;Hassenkam, Tue;Magnusson, Stig P.
通讯作者：
Magnusson, Stig P.
影响因子：
16.2
作者：
Bergmann, Olaf;Liebl, Jakob;Frisen, Jonas
通讯作者：
Frisen, Jonas
影响因子：
8.3
作者：
STUIVER, M;POLACH, HA
通讯作者：
POLACH, HA
DOI：
10.1034/j.1600-0838.2000.010006312.x
发表时间：
2000-12-01
影响因子：
4.1
作者：
Kannus, P
通讯作者：
Kannus, P