The organization of the leuC, leuD and leuB genes of the extreme thermophile Thermus thermophilus.

The organization of the leuC, leuD and leuB genes of the extreme thermophile Thermus thermophilus.
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极端嗜热栖热菌的 leuC、leuD 和 leuB 基因的组织。

DOI:
10.1016/s0378-1119(98)00482-x
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发表时间:
1998
期刊:
影响因子:
3.5
通讯作者:
T. Oshima
T. Oshima
中科院分区:
生物学3区
文献类型:
--
作者:
M. Tamakoshi;A. Yamagishi;T. Oshima

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3-leuB基因编码异丙基苹果酸脱氢酶,leuC和leuB基因分别编码亮氨酸生物合成途径中异丙基苹果酸异构酶的大亚基和小亚基。通过序列分析研究了极端嗜热菌Thermus thermophilus的leuB、leuC和leuD基因的组织结构。基因的位置也进行了测试的嗜热菌和大肠杆菌的亮氨酸缺乏的互补分析。该顺序是leuC、leuD和leuB基因,与以前的报道相反,它们彼此不重叠。leuC和leuD基因的序列分析表明,铁-硫结合的半胱氨酸残基和其他氨基酸残基参与异构酶活性,这已被推断从分析相关的蛋白质,乌头酸酶,是高度保守的。
3-Isopropylmalate dehydrogenase is encoded by leuB gene while leuC and leuB genes encode the large and small subunits of isopropylmalate isomerase in leucine biosynthetic pathway, respectively. Organization of the leuB, leuC and leuD genes of an extreme thermophile, Thermus thermophilus, was investigated by sequence analysis. Location of the genes was also tested by complementation analysis of leu deficiency of the thermophile and Escherichia coli. The order was the leuC, leuD, and leuB genes and, in contrast to a previous report, they did not overlap with each other. Sequence analysis of the leuC and leuD genes suggested that cysteine residues for iron–sulfur binding and other amino acid residues involved in isomerase activity, which have been inferred from analysis of a related protein, aconitase, were highly conserved.
问号钩端螺旋体波莫纳血清leuB基因的克隆与分析
DOI: 10.1099/00221287-139-5-1093
发表时间: 1993
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通讯作者: Yelton,DB
DOI: --
发表时间: 1980-08
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