Reduced expression levels of let-7c in human breast cancer patients.
Reduced expression levels of let-7c in human breast cancer patients.
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人类乳腺癌患者中 let-7c 的表达水平降低
DOI：
10.3892/ol.2015.2877
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发表时间：
2015-03
期刊：
影响因子：
2.9
通讯作者：
Xie SY
中科院分区：
文献类型：
--
作者：
Li XX;Gao SY;Wang PY;Zhou X;Li YJ;Yu Y;Yan YF;Zhang HH;Lv CJ;Zhou HH;Xie SY
循环中的微小RNA（miRNA）在许多疾病的诊断中非常重要，因为与从血液样本中分离的miRNA相比，血清或血浆miRNA更稳定。本研究旨在探讨乳腺癌患者血清let-7 c miRNA表达水平与临床诊断的关系。通过逆转录-定量聚合酶链反应测定90例BC患者和64例健康对照的循环let-7 c水平。结果表明，与癌旁对照组织相比，BC组织中let-7 c的表达下调。BC患者血清let-7 c表达水平明显低于健康对照组（P<0.01）。以0.374×103拷贝/ml为临界值，let-7 c的血清表达水平对区分BC患者和健康对照组的敏感性为87.5%，特异性为78.9%（受试者工作特征曲线下面积为0.848; 95%可信区间为0.785-0.911）。绝经前患者血清let-7 c表达水平显著高于绝经后患者（P<0.05），支持绝经状态可能影响let-7 c表达水平的假说。然而，ER（或PR）阳性和阴性患者之间的let-7 c血清水平无统计学显著差异。因此，目前的研究假设，血清let-7 c可以作为一种新的和有价值的生物标志物用于诊断BC。
Circulating microRNAs (miRNAs) are important in the diagnosis of a number of diseases, since serum or plasma miRNAs are more stable compared with miRNA isolated from blood samples. The aim of the present study was to investigate the association between the expression levels of serum let-7c miRNA and the clinical diagnosis of breast cancer (BC). The circulating let-7c levels of 90 BC patients and 64 healthy controls were determined by performing a reverse transcription-quantitative polymerase chain reaction assay. The results demonstrated that let-7c expression was downregulated in the BC tissues compared with the paracarcinoma control tissues. In addition, the let-7c expression in the serum of BC patients was significantly lower compared with the healthy controls (P<0.01). Using a cutoff value of 0.374×103 copies/ml, the serum expression levels of let-7c exhibited 87.5% sensitivity and 78.9% specificity for distinguishing BC patients from healthy controls (area under the receiver operating characteristic curve, 0.848; 95% confidence interval, 0.785–0.911). Furthermore, the results demonstrated that the serum expression levels of let-7c were significantly higher in premenopausal compared with postmenopausal patients (P<0.05), supporting the hypothesis that postmenopausal status may affect the serum expression levels of let-7c. However, no statistically significant differences were detected in the serum levels of let-7c between ER (or PR)-positive and -negative patients. Therefore, the current study hypothesized that serum let-7c may be used as a novel and valuable biomarker for the diagnosis of BC.
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影响因子：
1.8
作者：
Faheem M;Mahmood H;Khurram M;Qasim U;Irfan J
通讯作者：
Irfan J
DOI：
10.1186/bcr2766
发表时间：
2010
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Roth C;Rack B;Müller V;Janni W;Pantel K;Schwarzenbach H
通讯作者：
Schwarzenbach H
用于乳腺癌检测的血清全基因组 microRNA 分析
影响因子：
5
作者：
Wu, Qian;Wang, Chao;Ge, Qinyu
通讯作者：
Ge, Qinyu
影响因子：
25.7
作者：
Shimizu, Satoshi;Takehara, Tetsuo;Hayashi, Norio
通讯作者：
Hayashi, Norio
影响因子：
2.6
作者：
Macfarlane LA;Murphy PR
通讯作者：
Murphy PR