Array comparative hybridisation reveals a high degree of similarity between UK and European clinical isolates of hypervirulent Clostridium difficile.
Array comparative hybridisation reveals a high degree of similarity between UK and European clinical isolates of hypervirulent Clostridium difficile.
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DOI：
10.1186/1471-2164-11-389
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发表时间：
2010-06-21
期刊：
影响因子：
4.4
通讯作者：
Minton NP
中科院分区：
文献类型：
--
作者：
Marsden GL;Davis IJ;Wright VJ;Sebaihia M;Kuijper EJ;Minton NP
艰难梭菌是一种革兰氏阳性、厌氧、孢子形成的细菌，是人类艰难梭菌相关疾病的罪魁祸首，目前是西方世界医院腹泻的最常见原因。这一现状与出现高毒力pcr - 027型核型毒株有关。本工作的目的是通过阵列比较杂交鉴定可作为高毒力pcr -核型027菌株和已测序菌株CD630标记的序列分化区域。在这项研究中，我们通过阵列比较基因组杂交检测了从欧洲大陆和英国分离的94种最常见的pcr -核糖型临床菌株。我们的阵列是全面的，有40,097个寡核苷酸覆盖艰难梭菌630基因组，并揭示了32%的所有菌株的核心基因组。该阵列还覆盖了两种pcr -核糖型027菌株所特有的基因，而艰难梭菌630则由681个探针代表。所有这些基因也在常见的pcr -核型001和106以及新出现的高毒力pcr -核型078菌株中发现，表明这些基因是所有高毒力菌株的标记。我们通过鉴定CD630的标记物和与高毒力相关的标记物实现了本研究的目的，尽管这些基因不仅仅是pcr -核糖型027菌株的指示性基因。我们还扩展了这项研究，并定义了一个更严格的核心基因集，与之前发表的相比，由于全面的阵列覆盖。除此之外，我们还定义了非致毒菌株中缺失的基因列表，并定义了菌株CD37中特异性毒素缺失的性质。
Clostridium difficile is a Gram-positive, anaerobic, spore-forming bacterium that is responsible for C. difficile associated disease in humans and is currently the most common cause of nosocomial diarrhoea in the western world. This current status has been linked to the emergence of a highly virulent PCR-ribotype 027 strain. The aim of this work was to identify regions of sequence divergence that may be used as genetic markers of hypervirulent PCR-ribotype 027 strains and markers of the sequenced strain, CD630 by array comparative hybridisation. In this study, we examined 94 clinical strains of the most common PCR-ribotypes isolated in mainland Europe and the UK by array comparative genomic hybridisation. Our array was comprehensive with 40,097 oligonucleotides covering the C. difficile 630 genome and revealed a core genome for all the strains of 32%. The array also covered genes unique to two PCR-ribotype 027 strains, relative to C. difficile 630 which were represented by 681 probes. All of these genes were also found in the commonly occuring PCR-ribotypes 001 and 106, and the emerging hypervirulent PCR-ribotype 078 strains, indicating that these are markers for all highly virulent strains. We have fulfilled the aims of this study by identifying markers for CD630 and markers associated with hypervirulence, albeit genes that are not just indicative of PCR-ribotype 027 strains. We have also extended this study and have defined a more stringent core gene set compared to those previously published due to the comprehensive array coverage. Further to this we have defined a list of genes absent from non-toxinogenic strains and defined the nature of the specific toxin deletion in the strain CD37.
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影响因子：
3
作者：
Indra A;Huhulescu S;Schneeweis M;Hasenberger P;Kernbichler S;Fiedler A;Wewalka G;Allerberger F;Kuijper EJ
通讯作者：
Kuijper EJ
影响因子：
3.2
作者：
Janvilisri, Tavan;Scaria, Joy;Chang, Yung-Fu
通讯作者：
Chang, Yung-Fu
影响因子：
3
作者：
Allen-Vercoe, E;Woodward, MJ
通讯作者：
Woodward, MJ
影响因子：
3.5
作者：
Braun, V;Hundsberger, T;vonEichelStreiber, C
通讯作者：
vonEichelStreiber, C
影响因子：
4.5
作者：
Barbut, Frederic;Gariazzo, Beatrice;Petit, Jean-Claude
通讯作者：
Petit, Jean-Claude