Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping.
Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping.
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DOI：
10.1099/jmm.0.47714-0
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发表时间：
2008-11
影响因子：
3
通讯作者：
Kuijper EJ
中科院分区：
文献类型：
--
作者：
Indra A;Huhulescu S;Schneeweis M;Hasenberger P;Kernbichler S;Fiedler A;Wewalka G;Allerberger F;Kuijper EJ
我们建立了一种基于毛细管凝胶电泳的艰难梭菌PCR核糖体分型方法，并与传统的PCR核糖体分型方法进行了比较。共146个C。研究了艰难梭菌分离株：5个分离株是参考菌株（PCR核糖体型001、014、017、027和053）; 141个是临床分离株，包括2006-2007年期间在25个奥地利医疗机构收集的39个奥地利PCR核糖体型。毛细管凝胶电泳产生了高达11个片段，每个分离物和47核糖体型模式。除了一个参考菌株的5个PCR核糖核酸型都清楚地反映在基于毛细管分型的色谱图。毛细管凝胶电泳分为24个菌株属于PCR核糖型014分为7个亚组，而分型相同的菌株使用多位点可变数目串联重复分析产生三个不相关的亚组，没有明显的相关性SR亚组。使用基于网络的软件程序（http：webribo.ages.at），我们能够通过简单地将七个亚组模式分配给一个核糖体型，即PCR核糖体型014来正确地鉴定这些014分离物。我们认为基于毛细管凝胶电泳的PCR核糖分型是克服与分型结果的实验室间比较相关的问题的一种方法，同时大大减少了PCR核糖分型的动手时间。
We have developed a Clostridium difficile PCR ribotyping method based on capillary gel electrophoresis and have compared it with conventional PCR ribotyping. A total of 146 C. difficile isolates were studied: five isolates were reference strains (PCR ribotypes 001, 014, 017, 027 and 053); 141 were clinical isolates comprising 39 Austrian PCR ribotypes collected in the period 2006–2007 at 25 Austrian healthcare facilities. Capillary gel electrophoresis yielded up to 11 fragments per isolate and 47 ribotype patterns. All but one of the five PCR ribotypes of reference strains were clearly reflected in the chromatograms of capillary-based typing. Capillary gel electrophoresis divided 24 isolates belonging to PCR ribotype type 014 into seven subgroups, whereas subtyping the same isolates using multiple-locus variable-number tandem-repeat analysis yielded three unrelated subgroups, without obvious correlation to sr subgroups. Using a web-based software program (http://webribo.ages.at), we were able to correctly identify these 014 isolates by simply allocating the seven subgroup patterns to one ribotype, i.e. to PCR ribotype 014. We consider capillary gel electrophoresis-based PCR ribotyping to be a way of overcoming the problems associated with inter-laboratory comparisons of typing results, while at the same time substantially diminishing the hands-on time for PCR ribotyping.
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影响因子：
9.4
作者：
Lemee, L;Dhalluin, A;Pons, JL
通讯作者：
Pons, JL
影响因子：
2.1
作者：
Kato, H;Kato, N;Lyerly, DM
通讯作者：
Lyerly, DM
影响因子：
9.4
作者：
KATO, N;OU, CY;UENO, K
通讯作者：
UENO, K
影响因子：
11.8
作者：
McDonald, LC;Owings, M;Jernigan, DB
通讯作者：
Jernigan, DB
影响因子：
2.1
作者：
Stubbs, S;Rupnik, M;Popoff, M
通讯作者：
Popoff, M