A search for structurally similar cellular internal ribosome entry sites.
A search for structurally similar cellular internal ribosome entry sites.
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寻找结构相似的细胞内部核糖体进入位点。
DOI：
10.1093/nar/gkm483
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发表时间：
2007
影响因子：
14.9
通讯作者：
Holcik M
中科院分区：
文献类型：
--
作者：
Baird SD;Lewis SM;Turcotte M;Holcik M
内部核糖体进入位点(IRES)允许核糖体以帽不依赖的方式被招募到mRNA中。一些损害帽依赖翻译启动的病毒利用IRES来确保病毒RNA将有效地竞争翻译机制。IRES还被用于在抑制依赖于帽的翻译启动的条件下翻译蜂窝消息的子集。丙型肝炎病毒和猪瘟病毒的IRES具有相似的结构/功能，但没有共享初级序列相似性。到目前为止，在获得的细胞IRES结构中，没有一种结构具有总体结构相似性。因此，我们对人类5‘非翻译区进行了全基因组搜索，以寻找结构/功能相似的新的IRES，这些非翻译区的结构来自于关键的凋亡抑制因子X连锁的凋亡抑制蛋白(XIAP)。在这项搜索中发现的具有IRES活性的前三个匹配是Aquaporin 4、ELG1和NF-kappaB抑制因子(NRF)的5‘UTRs。AQP4和ELG1 IRES的结构与XIAP IRES的相似性有限，但它们有共同的结合XIAP IRES的反式作用因子。因此，我们认为细胞IRES不像病毒IRES那样由整体结构来定义，而是依赖于短基序和反式作用因子来发挥其功能。
Internal ribosome entry sites (IRES) allow ribosomes to be recruited to mRNA in a cap-independent manner. Some viruses that impair cap-dependent translation initiation utilize IRES to ensure that the viral RNA will efficiently compete for the translation machinery. IRES are also employed for the translation of a subset of cellular messages during conditions that inhibit cap-dependent translation initiation. IRES from viruses like Hepatitis C and Classical Swine Fever virus share a similar structure/function without sharing primary sequence similarity. Of the cellular IRES structures derived so far, none were shown to share an overall structural similarity. Therefore, we undertook a genome-wide search of human 5′UTRs (untranslated regions) with an empirically derived structure of the IRES from the key inhibitor of apoptosis, X-linked inhibitor of apoptosis protein (XIAP), to identify novel IRES that share structure/function similarity. Three of the top matches identified by this search that exhibit IRES activity are the 5′UTRs of Aquaporin 4, ELG1 and NF-kappaB repressing factor (NRF). The structures of AQP4 and ELG1 IRES have limited similarity to the XIAP IRES; however, they share trans-acting factors that bind the XIAP IRES. We therefore propose that cellular IRES are not defined by overall structure, as viral IRES, but are instead dependent upon short motifs and trans-acting factors for their function.
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影响因子：
5.4
作者：
HOFFMAN, MA;PALMENBERG, AC
通讯作者：
PALMENBERG, AC
影响因子：
4.8
作者：
Jang, GM;Leong, LEC;Semler, BL
通讯作者：
Semler, BL
影响因子：
5.3
作者：
Holcík, M;Gordon, BW;Korneluk, RG
通讯作者：
Korneluk, RG
影响因子：
4.5
作者：
Holcik, M;Graber, T;Baird, S
通讯作者：
Baird, S
影响因子：
14.9
作者：
Clamp, M;Andrews, D;Birney, E
通讯作者：
Birney, E